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pVG144

Plasmid

#227760

Purpose

Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Val121Met mutant

Promoter

PGK1pr

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG124

Plasmid

#227758

Purpose

Expresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Thr83Ser mutant

Promoter

PGK1pr

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOGS539_GLuc-ns

Plasmid

#226719

Purpose

Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

Depositor

Gautam Dantas

Publication

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Gaussia Luciferase

Tags

HA tag

Expression

Yeast

Promoter

pTEF1

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-NLS-3HA-KanMX6

Plasmid

#228022

Purpose

To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ6-HIS5

Plasmid

#228259

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

Depositor

Zhiping Xie

Publication

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

his5

Expression

Yeast

Promoter

TEF1 S.c.

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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CPY-ymsfGFP

Plasmid

#209538

Purpose

Expresses CYP tagged with ymsfGFP in S. cerevisiae

Depositor

Patrick Lajoie

Publication

Chadwick et al Mol Biol Cell. 2024 Dec 11:mbcE24040147.

Insert

CPY

Tags

ymsfGFP

Expression

Yeast

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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CPY*-ymsfGFP

Plasmid

#209539

Purpose

Expresses the misfolded CPY* mutant tagged with ymsfGFP in S. cerevisiae

Depositor

Patrick Lajoie

Publication

Chadwick et al Mol Biol Cell. 2024 Dec 11:mbcE24040147.

Insert

CPY*

Tags

ymsfGFP

Expression

Yeast

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-3HA-HIS3MX6

Plasmid

#228025

Purpose

To integrate TID-FKBP fusion at yeast promoter; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-NLS-3HA-HISMX6

Plasmid

#228027

Purpose

To integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with HISMX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-ARG (V5 and HA)

Plasmid

#222493

Purpose

Co-expression of two genes in yeast with V5 and HA tags and ARG1 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Publication

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ10-LEU2

Plasmid

#228262

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Publication

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

LEU2

Expression

Yeast

Promoter

LEU2 K.l.

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-TID-FKBP-HA3

Plasmid

#227998

Purpose

Expresses TurboID-FKBP fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-HIS3p-TID-FKBP-HA3

Plasmid

#228006

Purpose

Expresses TurboID-FKBP fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

HIS3

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46-His (SB250)

Plasmid

#227599

Purpose

Inducible expression of His-tagged SLC25A46 in Pichia pastoris

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Promoter

AOX1

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46[I349D]-His (SB256)

Plasmid

#227605

Purpose

Inducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastoris

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Mutation

I349D

Promoter

AOX1

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1 (SB251)

Plasmid

#227600

Purpose

Inducible expression of Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-3HA-KanMX6

Plasmid

#228024

Purpose

To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-NLS-3HA-KanMX6

Plasmid

#228026

Purpose

To integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BASU-HA3-KanMX6

Plasmid

#227978

Purpose

To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU

Tags

HA tag

Expression

Yeast

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BioID2-HA3-KanMX6

Plasmid

#227976

Purpose

To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Publication

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID2

Tags

HA tag

Expression

Yeast

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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