8,060 results

1. AtPIN1

   Plasmid

   #117088

   Purpose

   protein expression in yeast

   Depositor

   Geoffrey Chang

   Insert

   AtPIN1
   

   Use

   Tags

   His

   Expression

   Yeast

   Mutation

   Promoter

   AOX

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGBW-m4046248

   Plasmid

   #153865

   Purpose

   Yeast expression plasmid for SARS-CoV-2 helicase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pRS426-ACT_unstable

   Plasmid

   #127608

   Purpose

   high-copy URA-selectable plasmid, WT ACT1 intron (unstable) inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   Unstable ACT1 intron (WT)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS426-ECM_stable

   Plasmid

   #127606

   Purpose

   high-copy URA-selectable plasmid, WT ECM33 intron (stable) inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   Stable ECM33 intron (WT)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. piHGpd/p82

   Plasmid

   #1105

   Depositor

   Susan Lindquist

   Article

   Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

   Insert

   HSP82 (HSP82 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wild-type HSP82 regulated by the strong constitut…

   Promoter

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pSec7-iGFPx6

   Plasmid

   #115424

   Purpose

   Express Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesis

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   Sec7-iGFPx6
   

   Use

   Tags

   iGFP

   Expression

   Yeast

   Mutation

   Promoter

   Endogenous

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 416Gal-FUS-R524T-YFP

   Plasmid

   #29627

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   R524T

   Promoter

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pGBW-m4046369

   Plasmid

   #149118

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp2

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046382

   Plasmid

   #145570

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4046593

    Plasmid

    #145539

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046376

    Plasmid

    #145481

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pES1002

    Plasmid

    #21789

    Depositor

    Richard Yu

    Article

    Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

    Insert

    Fus3-as2 (FUS3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    contains 5' 1kb upstream UTR/promoter Q93…

    Promoter

    Available Since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 416Gal-FUS-R518K-YFP

    Plasmid

    #29621

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R518K

    Promoter

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

14. CDC12(WT)

    Plasmid

    #1162

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    CDC12 (CDC12 Budding Yeast)
    

    Use

    Tags

    266bp of upstream sequence

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBSWHhc

    Plasmid

    #133901

    Purpose

    Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4046342

    Plasmid

    #149077

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pAG413GPD-ccdB-TAP

    Plasmid

    #14262

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBW-m4046699

    Plasmid

    #149153

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046393

    Plasmid

    #149132

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. Gal ts-lux

    Plasmid

    #1329

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

    Insert

    bacterial luciferase-V.h.MAV
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only