8,126 results

1. pAG415Gal_Gcn4 F124IHA

   Plasmid

   #232993

   Purpose

   Galactose iduced expression of Gcn4 F124IHA in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 F124I
   

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   F124I

   Promoter

   Gal1

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAG415Gal_FL Gcn4

   Plasmid

   #232955

   Purpose

   Galactose iduced expression of full-length Gcn4 in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Fyv6-NFLAG del1-16

   Plasmid

   #234138

   Purpose

   First 16 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Mutation

   N-terminus truncation

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Fyv6-NFLAG del103-173

   Plasmid

   #234134

   Purpose

   Amino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminus

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Mutation

   C-terminus truncation

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS414-Fyv6-CFLAG

   Plasmid

   #234133

   Purpose

   Restriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Fyv6-NFLAG del134-173

   Plasmid

   #234135

   Purpose

   Amino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminus

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Mutation

   C-terminus truncation

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS414-Fyv6

   Plasmid

   #234140

   Purpose

   Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS414

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS414-NFLAG-Fyv6

   Plasmid

   #234139

   Purpose

   Restriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414

   Depositor

   Aaron Hoskins

   Insert

   Fyv6
   

   Expression

   Yeast

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS424 Prp8 G1868R

   Plasmid

   #234124

   Purpose

   Suppressor screen mutant in full-length Prp8 on a TRP plasmid

   Depositor

   Aaron Hoskins

   Insert

   Prp8
   

   Expression

   Yeast

   Mutation

   G1868R

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS416-Fyv6

    Plasmid

    #234141

    Purpose

    Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS416

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Expression

    Yeast

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Fyv6-NFLAG del1-23

    Plasmid

    #234137

    Purpose

    First 23 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Expression

    Yeast

    Mutation

    N-terminus truncation

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS314-Cef1-M175I

    Plasmid

    #234118

    Purpose

    Suppressor screen mutant in Cef2

    Depositor

    Aaron Hoskins

    Insert

    Cef1
    

    Expression

    Yeast

    Mutation

    M175I

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS314-Cef1-Q193P

    Plasmid

    #234117

    Purpose

    Suppressor screen mutant in Cef1

    Depositor

    Aaron Hoskins

    Insert

    Cef1
    

    Expression

    Yeast

    Mutation

    Q193P

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS314-Cef1-A37V

    Plasmid

    #234119

    Purpose

    Suppressor screen mutant in Cef3

    Depositor

    Aaron Hoskins

    Insert

    Cef1
    

    Expression

    Yeast

    Mutation

    A37V

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS416-Syf1

    Plasmid

    #234113

    Purpose

    Syf1 region PCR, restriction digest and ligation into pRS416 backbone

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Expression

    Yeast

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS414-Syf1 634 Truncation

    Plasmid

    #234116

    Purpose

    Syf1 PCR truncation starting at 634 through the end of the gene

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Expression

    Yeast

    Mutation

    N-terminus truncation

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGADT7_Sr62NLRa

    Plasmid

    #233528

    Purpose

    Expresses Sr62NLRa in yeast

    Depositor

    Brande Wulff

    Article

    Chen et al bioRxiv 2024.08.30.610287

    Insert

    Sr62NLRa
    

    Tags

    GAL4 AD, HA tag

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS414-Syf1

    Plasmid

    #234114

    Purpose

    Syf1 region PCR, restriction digest and ligation into pRS414 backbone

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Expression

    Yeast

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS414-Syf1 778 Truncation

    Plasmid

    #234115

    Purpose

    Syf1 PCR truncation starting at 778 through the end of the gene

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Expression

    Yeast

    Mutation

    N-terminus truncation

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG415Gal_Gcn4 WTSLFD

    Plasmid

    #232980

    Purpose

    Galactose iduced expression of Gcn4 WTSLFD+ in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WTSLFD+
    

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    F108W, E109T, Y110S, E111L, N112F, L113D

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge