8,060 results

1. pML107-VHT1

   Plasmid

   #232898

   Purpose

   Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   VHT1 gRNA (VHT1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pML104-HIS3-2

   Plasmid

   #232881

   Purpose

   Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   HIS3 gRNA (HIS3 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pML107-ADE13

   Plasmid

   #232887

   Purpose

   Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   ADE13 gRNA (ADE13 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pML107-LAS17

   Plasmid

   #232892

   Purpose

   Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   LAS17 gRNA (LAS17 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pML104-LEU2-2

   Plasmid

   #232883

   Purpose

   Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   LEU2 gRNA (LEU2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS313-rad53-5004

   Plasmid

   #232902

   Purpose

   Plasmid carrying the rad53-5004 allele with its native promoter and terminator.

   Depositor

   Jolanda Van Leeuwen

   Insert

   RAD53 (RAD53 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T600C, A680G, A1094T, C1615T, C2228A

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pML107-INO80

   Plasmid

   #232890

   Purpose

   Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   INO80 gRNA (INO80 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCas9-EcRT-Z3-Nat

   Plasmid

   #232094

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-FLAG-APEX2-NES-K.l.LEU2

   Plasmid

   #229229

   Purpose

   Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

   Depositor

   Fulvio Reggiori

   Article

   Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-FLAG-APEX2-NES-NatMX6

    Plasmid

    #229231

    Purpose

    Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

    Depositor

    Fulvio Reggiori

    Article

    Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-FLAG-APEX2-NES-TRP1

    Plasmid

    #229228

    Purpose

    Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

    Depositor

    Fulvio Reggiori

    Article

    Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PAF23

    Plasmid

    #223171

    Purpose

    Expresses yeGFP under control of CTR1 promoter and terminator

    Depositor

    Ryan Peterson

    Article

    Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

    Insert

    green fluorescent protein
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Saccharomyces cerevisiae CTR1

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PAF24

    Plasmid

    #223172

    Purpose

    Expresses VC83_00191-yeGFP under control of CTR1 promoter and terminator

    Depositor

    Ryan Peterson

    Article

    Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

    Insert

    Copper Transporter
    

    Use

    Tags

    StreptagII and yeGFP

    Expression

    Yeast

    Mutation

    Promoter

    saccharomyces cerevisiae CTR1

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDRF1 ymScarletI3

    Plasmid

    #224106

    Purpose

    Expressing ymScarletI3 in yeast

    Depositor

    Bas Teusink

    Insert

    ymScarletI3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. P415 ymiRFP680

    Plasmid

    #224109

    Purpose

    Expressing ymiRFP680 in yeast

    Depositor

    Bas Teusink

    Insert

    ymiRFP680
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. P415 ymScarletI3

    Plasmid

    #224111

    Purpose

    Expressing ymScarletI3 in yeast

    Depositor

    Bas Teusink

    Insert

    ymScarletI3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-link-ymBaojin-URA3

    Plasmid

    #224081

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mBaojin with URA3 selection

    Depositor

    Bas Teusink

    Insert

    ymBaoJin
    

    Use

    Tags

    ymBaoJin

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDRF1 ymBaojin

    Plasmid

    #224085

    Purpose

    Expressing ymBaojin in yeast

    Depositor

    Bas Teusink

    Insert

    ymBaoJin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. P415 ymTurquoise2

    Plasmid

    #224093

    Purpose

    Expressing ymTurquoise2 in yeast

    Depositor

    Bas Teusink

    Insert

    ymTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-link-yemiRFP670-SpHis5

    Plasmid

    #224098

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with SpHis5 selection

    Depositor

    Bas Teusink

    Insert

    yemiRFP670
    

    Use

    Tags

    yemiRFP670

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge