8,113 results

1. pCfb12567

   Plasmid

   #219927

   Purpose

   PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

   Depositor

   Irina Borodina

   Insert

   Promoter CYC
   

   Expression

   Yeast

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfb12566

   Plasmid

   #219926

   Purpose

   PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

   Depositor

   Irina Borodina

   Insert

   Promoter DGA
   

   Expression

   Yeast

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLS406

   Plasmid

   #231078

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9-EcRT-Z3-Kan

   Plasmid

   #232096

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Z3

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfb13236

   Plasmid

   #219909

   Purpose

   The base plasmid of TUNEYALI for TF46

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF46 ( YALI1_E20229g) and homologous arm matching TF46
   

   Expression

   Yeast

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLS405

   Plasmid

   #231077

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker LEU2

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLS404

   Plasmid

   #231076

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker TRP1

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLS408

   Plasmid

   #231079

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker natMX6

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLS403

   Plasmid

   #231075

   Purpose

   Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker HIS3

   Depositor

   Sahand Jamal Rahi

   Article

   Scutteri et al bioRxiv 2024.11.05.622133

   Type

   Empty backbone

   Use

   Integrating

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLS400

    Plasmid

    #231080

    Purpose

    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX

    Depositor

    Sahand Jamal Rahi

    Article

    Scutteri et al bioRxiv 2024.11.05.622133

    Type

    Empty backbone

    Use

    Integrating

    Expression

    Bacterial and Yeast

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pdnCas3-CDA1

    Plasmid

    #229535

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pdnCas3-APOBEC1

    Plasmid

    #229537

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas3-APOBEC1

    Plasmid

    #229536

    Purpose

    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCas3-CDA1

    Plasmid

    #229534

    Purpose

    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfb13199

    Plasmid

    #219874

    Purpose

    The base plasmid of TUNEYALI forTF09

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09
    

    Expression

    Yeast

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCfb13218

    Plasmid

    #219893

    Purpose

    The base plasmid of TUNEYALI for TF28

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28
    

    Expression

    Yeast

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETcon-pGAL1-Aga2-α-Synuclein-c_myc

    Plasmid

    #225222

    Purpose

    Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

    Inserts

    α-Synuclein
    Aga2
    

    Tags

    GS linker, HA Tag, and c-myc Tag

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

    Plasmid

    #225228

    Purpose

    Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

    Inserts

    Aga2
    Htt (25Q)
    

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

    Plasmid

    #225229

    Purpose

    Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

    Inserts

    Aga2
    Htt (46Q)
    

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS316-SSD1-L1374

    Plasmid

    #226278

    Purpose

    Plasmid expressing the SSD1 allele from L-1374, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SSD1 (SSD1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge