8,103 results

1. pRS416-MET25p-TID-FRB-NLS-HA3

   Plasmid

   #228017

   Purpose

   Expresses TurboID-FRB-NLS fusion from MET25 promoter; yeast BioID

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID-FRB
   

   Tags

   HA tag

   Expression

   Yeast

   Promoter

   MET25

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS416-MET25p-TID-FKBP-HA3

   Plasmid

   #228018

   Purpose

   Expresses TurboID-FKBP fusion from MET25 promoter; yeast BioID

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID-FKBP
   

   Tags

   HA tag

   Expression

   Yeast

   Promoter

   MET25

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS416-MET25p- TID-FKBP-NLS-HA3

   Plasmid

   #228019

   Purpose

   Expresses TurboID-FKBP-NLS fusion from MET25 promoter; yeast BioID

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID-FKBP
   

   Tags

   HA tag

   Expression

   Yeast

   Promoter

   MET25

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPICZ A-SLC25A46-His (SB250)

   Plasmid

   #227599

   Purpose

   Inducible expression of His-tagged SLC25A46 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Tags

   10xHis

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ A-SLC25A46[I349D]-His (SB256)

   Plasmid

   #227605

   Purpose

   Inducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   I349D

   Promoter

   AOX1

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPICZ A-SLC25A46[R247A,R347A]-His (SB257)

   Plasmid

   #227606

   Purpose

   Inducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   R247A,R347A

   Promoter

   AOX1

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPICZ A-MFN2-Strep (SB253)

   Plasmid

   #227602

   Purpose

   Inducible expression of Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   MFN2 (MFN2 Human)
   

   Tags

   Twin-StrepTag, 10xHis

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPICZ A-Strep-Opa1(MGD) (SB252)

   Plasmid

   #227601

   Purpose

   Inducible expression of Strep-tagged Opa1(MGD) in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   OPA1 (OPA1 Human)
   

   Tags

   Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 253-580 (MGD) and aa 938-960

   Promoter

   AOX1

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfb13247

   Plasmid

   #219918

   Purpose

   The base plasmid of TUNEYALI for TF57

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57
   

   Expression

   Yeast

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCfb13228

    Plasmid

    #219903

    Purpose

    The base plasmid of TUNEYALI for TF38

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38
    

    Expression

    Yeast

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCfb13210

    Plasmid

    #219885

    Purpose

    The base plasmid of TUNEYALI for TF20

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20
    

    Expression

    Yeast

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFa6-TID-FKBP-3HA-KanMX6

    Plasmid

    #228024

    Purpose

    To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFa6-TID-FKBP-NLS-3HA-KanMX6

    Plasmid

    #228026

    Purpose

    To integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-BASU-HA3-KanMX6

    Plasmid

    #227978

    Purpose

    To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BASU
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-BioID2-HA3-KanMX6

    Plasmid

    #227976

    Purpose

    To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BioID2
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-BirA-HA3-KanMX6

    Plasmid

    #227974

    Purpose

    To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BirA
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

    Plasmid

    #227084

    Purpose

    Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus

    Depositor

    Chris Hill

    Insert

    spt6-tsh2 (SPT6 Budding Yeast)
    

    Tags

    STREPii-FLAG and SV40 NLS 1

    Expression

    Yeast

    Promoter

    Gal1-10

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CUP1-∆ssCPY*-mScarletI

    Plasmid

    #221157

    Purpose

    Fluorescent reporter for cytoplasmic aggregates

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Tags

    mScarletI

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CUP1-∆ssCPY*-mNeonGreen

    Plasmid

    #221156

    Purpose

    Fluorescent reporter for cytoplasmic aggregates

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CUP1-∆ssCPY*-mNeonGreen in pRS315

    Plasmid

    #221160

    Purpose

    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

    Depositor

    Mark Leake

    Insert

    Carboxypeptidase Y (PRC1 Budding Yeast)
    

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Deletion signal peptide, G255A mutation

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge