8,060 results

1. RT-5

   Plasmid

   #220442

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (puf-3 Nematode)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RT-4

   Plasmid

   #220443

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (PUF3 Budding Yeast)
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MAP80A_MS2

   Plasmid

   #220624

   Purpose

   tRNA reporter vector

   Depositor

   Marvin Wickens

   Article

   Preston et al Nat Methods. 2019 May;16(5):437-445. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Leu2 2u

   Plasmid

   #220622

   Purpose

   empty backbone for shuttle vector construction

   Depositor

   Marvin Wickens

   Article

   Preston et al Nat Methods. 2019 May;16(5):437-445. doi

   Insert

   empty vector (LEU2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RT-1

   Plasmid

   #220434

   Purpose

   Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pReporter_ZF2_ZF1_mNeonGreen

   Plasmid

   #213834

   Purpose

   Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pReporter_ZF1_mNeonGreen_ZF2

   Plasmid

   #213835

   Purpose

   Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pReporter_ZF1_ZF2_mNeonGreen

   Plasmid

   #213836

   Purpose

   Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pReporter_ZF2_mNeonGreen_ZF1

   Plasmid

   #213837

   Purpose

   Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pReporter_mNeonGreen

    Plasmid

    #213838

    Purpose

    Facilitates genomic integration of the control reporter without zinc finger binding sites.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPtet-1_mNeonGreen_Ptet-2_mCherry

    Plasmid

    #213839

    Purpose

    Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen, mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pReporter_ZF1_mNeonGreen

    Plasmid

    #213840

    Purpose

    Facilitates genomic integration of the reporter with upstream binding sites for ZF-1

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pReporter_ZF2_mNeonGreen

    Plasmid

    #213841

    Purpose

    Facilitates genomic integration of the reporter with upstream binding sites for ZF-2

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPtet-2-VP16

    Plasmid

    #213842

    Purpose

    Used for expressing VP16 fused to ZF-2.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPtet-1-VP16

    Plasmid

    #213843

    Purpose

    Used for expressing VP16 fused to ZF-1.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pStorage_Ptet-1_ZF1

    Plasmid

    #213844

    Purpose

    CombiGEM storage plasmid for ZF-1 CR fusions.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pStorage_Ptet-2_ZF2

    Plasmid

    #213845

    Purpose

    CombiGEM storage plasmid for ZF-2 CR fusions.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pReporter_TetR

    Plasmid

    #213847

    Purpose

    Facilitates genomic integration of tetR expression cassette.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    TetR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfb12622

    Plasmid

    #219929

    Purpose

    PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter ACT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfb13237

    Plasmid

    #219910

    Purpose

    The base plasmid of TUNEYALI for TF47

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge