8,108 results

1. pNK7188

   Plasmid

   #219768

   Purpose

   Vector for expression Pv4CL1 in yeast (P.pastoris)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   Pv4CL1
   

   Expression

   Yeast

   Promoter

   pGAP

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfb13220

   Plasmid

   #219895

   Purpose

   The base plasmid of TUNEYALI for TF30

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30
   

   Expression

   Yeast

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDRF1-GW mKelly-T2A-mtq2

   Plasmid

   #205506

   Purpose

   Expression of mKelly-T2A-mtq2 in yeast

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2025.06.27.661975; doi: https://

   Insert

   mKelly-T2A-mtq2
   

   Expression

   Yeast

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG426-GAL-APP-CTF

   Plasmid

   #181732

   Purpose

   Galactose inducible expression of APP-CTF

   Depositor

   Alejandro Chavez

   Insert

   APP-CTF (APP Human)
   

   Expression

   Yeast

   Mutation

   C-terminal fragment (aa 671-770)

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWR95(URA3)

   Plasmid

   #203917

   Purpose

   BLINCAR plasmid for gene deletion, URA3 target

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   URA3 upstream homology
   URA3 downstream homology
   

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWR91

   Plasmid

   #203915

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Insert

   CRF1 plus flanking (CRF1 S. stipitis (yeast))
   

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWR90

   Plasmid

   #203914

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Insert

   GSC2 plus flanking (GSC2 S. stipitis (yeast))
   

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWR89

   Plasmid

   #203423

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Insert

   EGC3 plus flanking (EGC3 S. stipitis (yeast))
   

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWR88

   Plasmid

   #203422

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, no genomic target

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   CAR copy 1
   P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
   CAR copy 2
   

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWR63(YUM1)

    Plasmid

    #203336

    Purpose

    episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    YUM1-targeting sequence
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWR63

    Plasmid

    #203331

    Purpose

    episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWR23

    Plasmid

    #202770

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coKanMX
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWR10

    Plasmid

    #202710

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coShBle
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWR9

    Plasmid

    #202645

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYES2/NTA/Leu-LegA7

    Plasmid

    #216518

    Purpose

    Expression of LegA7

    Depositor

    Ralph Isberg

    Insert

    Leg A7
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 WT

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYES2/NTA/Leu-LegA7Δank

    Plasmid

    #216519

    Purpose

    Expression of LegA7 ΔAnk

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 331-454

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYES2/NTA/Leu-LegA7ΔAnk290

    Plasmid

    #216520

    Purpose

    Expression of LegA7 ΔAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk290
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-454

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYES2/NTA/Leu-LegA7ΔNAnk264

    Plasmid

    #216521

    Purpose

    Expression of LegA7 ΔNAnk264

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk264
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 264-361

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYES2/NTA/Leu-LegA7ΔNAnk290

    Plasmid

    #216522

    Purpose

    Expression of LegA7 ΔNAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk290
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-361

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. RT-3

    Plasmid

    #220440

    Purpose

    Base construct used to create the PUF3/PUP2 tagging strain. (aka pCS2/3HA/PUM1_RBD/PUP2/URA3)

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    MV IE93 promoter

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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