Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

8,405 results

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pAG415Gal_DBDGFP

Plasmid

#232999

Purpose

Galactose iduced expression of Gcn4 DBDGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

DBD

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoAS

Plasmid

#232959

Purpose

Galactose iduced expression of Gcn4 ILVtoAS in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merGFP

Plasmid

#233000

Purpose

Galactose iduced expression of Gcn4 WT 44merGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 30mer

Plasmid

#232986

Purpose

Galactose iduced expression of Gcn4 WT 30mer in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 30mer

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRS424 Prp8 WT TRP1

Plasmid

#234120

Purpose

The N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORF

Depositor

Aaron Hoskins

Insert

Prp8

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ARB1

Plasmid

#232888

Purpose

Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ARB1 gRNA (ARB1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-CUP1pr-NFAPoptim

Plasmid

#221110

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-CUP1pr-CFAPoptim

Plasmid

#221111

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3

Plasmid

#221114

Purpose

FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST

Plasmid

#221118

Purpose

FAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3-deltaPEST

Expression

Yeast

Mutation

STE3 mutant that lacks the PEST domain in the C-t…

Promoter

STE3

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-K424R

Plasmid

#221119

Purpose

FAP tagged STE3-K424R mutant (N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3-K424R

Expression

Yeast

Mutation

STE3 mutantK424R

Promoter

STE3

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ANP1-FAPoptim

Plasmid

#221120

Purpose

FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ANP1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ERG6-FAPoptim

Plasmid

#221121

Purpose

FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ERG6-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-PMA1-FAPoptim

Plasmid

#221122

Purpose

FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

PMA1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-RPA34-FAPoptim

Plasmid

#221123

Purpose

FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

RPA34-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC7-FAPoptim

Plasmid

#221124

Purpose

FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC7-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-VPH1-FAPoptim

Plasmid

#221125

Purpose

FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

VPH1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-NFAPoptim

Plasmid

#221088

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-TEF1pr-CFAPoptim

Plasmid

#221089

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-NFAPorig

Plasmid

#221091

Purpose

FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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