8,110 results

1. pYES2/NTA/Leu-LegA7ΔNAnk290

   Plasmid

   #216522

   Purpose

   Expression of LegA7 ΔNAnk290

   Depositor

   Ralph Isberg

   Insert

   LegA7 ΔNAnk290
   

   Tags

   HIS-tag

   Expression

   Bacterial and Yeast

   Mutation

   LegA7 missing aa 290-361

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RT-3

   Plasmid

   #220440

   Purpose

   Base construct used to create the PUF3/PUP2 tagging strain. (aka pCS2/3HA/PUM1_RBD/PUP2/URA3)

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   MV IE93 promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. RT-2

   Plasmid

   #220438

   Purpose

   Base construct used to create PUP2-DADA/3HA fusion proteins in S. cerevisiae. (aka pBSII/PUP2-DADA/3HA/URA3)

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. RT-5

   Plasmid

   #220442

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (puf-3 Nematode)
   

   Expression

   Yeast

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. RT-4

   Plasmid

   #220443

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (PUF3 Budding Yeast)
   

   Tags

   3xHA

   Expression

   Yeast

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAP80A_MS2

   Plasmid

   #220624

   Purpose

   tRNA reporter vector

   Depositor

   Marvin Wickens

   Article

   Preston et al Nat Methods. 2019 May;16(5):437-445. doi

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Leu2 2u

   Plasmid

   #220622

   Purpose

   empty backbone for shuttle vector construction

   Depositor

   Marvin Wickens

   Article

   Preston et al Nat Methods. 2019 May;16(5):437-445. doi

   Insert

   empty vector (LEU2 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. RT-1

   Plasmid

   #220434

   Purpose

   Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pReporter_ZF2_ZF1_mNeonGreen

   Plasmid

   #213834

   Purpose

   Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

   Depositor

   James Collins

   Article

   Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

   Insert

   mNeonGreen
   

   Expression

   Yeast

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pReporter_ZF1_mNeonGreen_ZF2

    Plasmid

    #213835

    Purpose

    Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pReporter_ZF1_ZF2_mNeonGreen

    Plasmid

    #213836

    Purpose

    Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pReporter_ZF2_mNeonGreen_ZF1

    Plasmid

    #213837

    Purpose

    Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pReporter_mNeonGreen

    Plasmid

    #213838

    Purpose

    Facilitates genomic integration of the control reporter without zinc finger binding sites.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPtet-1_mNeonGreen_Ptet-2_mCherry

    Plasmid

    #213839

    Purpose

    Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen, mCherry
    

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pReporter_ZF1_mNeonGreen

    Plasmid

    #213840

    Purpose

    Facilitates genomic integration of the reporter with upstream binding sites for ZF-1

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pReporter_ZF2_mNeonGreen

    Plasmid

    #213841

    Purpose

    Facilitates genomic integration of the reporter with upstream binding sites for ZF-2

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    mNeonGreen
    

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPtet-2-VP16

    Plasmid

    #213842

    Purpose

    Used for expressing VP16 fused to ZF-2.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    VP16
    

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPtet-1-VP16

    Plasmid

    #213843

    Purpose

    Used for expressing VP16 fused to ZF-1.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Insert

    VP16
    

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pStorage_Ptet-1_ZF1

    Plasmid

    #213844

    Purpose

    CombiGEM storage plasmid for ZF-1 CR fusions.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pStorage_Ptet-2_ZF2

    Plasmid

    #213845

    Purpose

    CombiGEM storage plasmid for ZF-2 CR fusions.

    Depositor

    James Collins

    Article

    Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge