8,113 results

1. pToxamEXP4CUP1

   Plasmid

   #218284

   Purpose

   Use together with pJE13HD7 and pJE13HD7 derivatives to integrate RelE expression cassette under the control of the CUP1 promoter at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   Hph>tAgTEF1-ISceI-Repeat2-pCUP1>RelE(1-125)>RPL28intron>RelE(126, 289)>tTPI1-HO(1828, 1979)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pITEdv1

   Plasmid

   #218283

   Purpose

   Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pITEu2

   Plasmid

   #218282

   Purpose

   Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJE13HD7

   Plasmid

   #218281

   Purpose

   Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pILGFP4QE2Crimson

   Plasmid

   #218280

   Purpose

   Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRHDGAL43C

   Plasmid

   #218279

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRGAL43C

   Plasmid

   #218278

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRGAL4A

   Plasmid

   #218277

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIRHDGAL3C

   Plasmid

   #218276

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIRGAL3C

    Plasmid

    #218275

    Purpose

    Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPOM014_P2_pGPM1

    Plasmid

    #216439

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter gpm1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    May 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRH3128

    Plasmid

    #210165

    Purpose

    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ADE1-GFP::tADH1

    Depositor

    Randy Hampton

    Article

    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

    Insert

    YIp ADE2-LEU2 pTDH3::ADE1-GFP::tADH1 (ADE2 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRH2941

    Plasmid

    #210143

    Purpose

    Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1

    Depositor

    Randy Hampton

    Article

    Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

    Insert

    YIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1 (ARO7 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG426-GAL-Prepro-3x-Beta-Amyloid

    Plasmid

    #181730

    Purpose

    Galactose inducible expression of Prepro-3x-Beta-Amyloid

    Depositor

    Alejandro Chavez

    Insert

    Prepro-3x-Beta-Amyloid
    

    Expression

    Yeast

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG426-GAL-Prepro-3x-Adan

    Plasmid

    #181733

    Purpose

    Galactose inducible expression of Prepro-3x-Adan

    Depositor

    Alejandro Chavez

    Insert

    Prepro-3x-Adan
    

    Expression

    Yeast

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG416-GAL-TDP-43-M337V

    Plasmid

    #181721

    Purpose

    Galactose inducible expression of TDP-43-M337V

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Mutation

    M337V

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG416-GAL-TDP-43-N267S

    Plasmid

    #181720

    Purpose

    Galactose inducible expression of TDP-43-N267S

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Mutation

    N267S

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG416-GAL-TDP-43-Q331K

    Plasmid

    #181719

    Purpose

    Galactose inducible expression of TDP-43-Q331K

    Depositor

    Alejandro Chavez

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Mutation

    Q331K

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG416-GAL-FUS-R521C

    Plasmid

    #181718

    Purpose

    Galactose inducible expression of FUS-R521C

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Expression

    Yeast

    Mutation

    R521C

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG416-GAL-FUS-P431L

    Plasmid

    #181717

    Purpose

    Galactose inducible expression of FUS-P431L

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Expression

    Yeast

    Mutation

    P431L

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge