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pVG137

Plasmid

#227751

Purpose

Expresses a version of light-inducible transcription factor EL222 (Val201Leu) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Val201Leu mutant

Promoter

PGK1pr

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVG136

Plasmid

#227750

Purpose

Expresses a version of light-inducible transcription factor EL222 (Pro165Ser) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Pro165Ser mutant

Promoter

PGK1pr

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVG135

Plasmid

#227749

Purpose

Expresses a version of light-inducible transcription factor EL222 (Lys99Glu) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Lys99Glu mutant

Promoter

PGK1pr

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVG119

Plasmid

#227748

Purpose

Expresses a version of light-inducible transcription factor EL222 (Val139Leu) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Val139Leu mutant

Promoter

PGK1pr

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVG118

Plasmid

#227747

Purpose

Expresses a version of light-inducible transcription factor EL222 (Asp31Tyr) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Asp18Tyr mutant

Promoter

PGK1pr

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVG117

Plasmid

#227746

Purpose

Expresses a version of light-inducible transcription factor EL222 (Asp31Tyr, Val139Leu) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Asp18Tyr, Val139Leu double mutant

Promoter

PGK1pr

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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YIplac128-TEF2p-LDH-CYC1t

Plasmid

#216120

Purpose

Yeast integrative vector for constitutive expression of LDH at the LEU2 locus

Depositor

Yvonne Nygård

Publication

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Lactate dehydrogenase (LDH Bovine)

Expression

Yeast

Promoter

TEF2 promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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YIplac204-TDH3p-LDH-CYC1t

Plasmid

#216121

Purpose

Yeast integrative vector for constitutive expression of LDH at the TRP1 locus

Depositor

Yvonne Nygård

Publication

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Lactate dehydrogenase (LDH Bos taurus, Bovine)

Expression

Yeast

Promoter

TDH3 promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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YIplac204-TDH3p-CDC19-CYC1t

Plasmid

#216122

Purpose

Yeast integrative vector for constitutive expression of CDC19 at the TRP1 locus

Depositor

Yvonne Nygård

Publication

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Pyruvate kinase 1

Expression

Yeast

Promoter

TDH3 promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMY.002

Plasmid

#228681

Purpose

pCCW12 Promoter for yeast expression with Nterminal tag

Depositor

Chen Davidovich

Publication

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

pCCW12

Expression

Yeast

Mutation

WT

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pOGS539_GLuc

Plasmid

#226718

Purpose

Plasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)

Depositor

Gautam Dantas

Publication

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Gaussia Luciferase

Tags

HA tag and Mating factor alpha secretion signal

Expression

Yeast

Promoter

pTEF1

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMY.007

Plasmid

#228686

Purpose

pGAL1 Promoter for yeast expression with Nterminal tag

Depositor

Chen Davidovich

Publication

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

pGAL1

Expression

Yeast

Mutation

WT

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMY.006

Plasmid

#228685

Purpose

pTEF2 Promoter for yeast expression with Nterminal tag

Depositor

Chen Davidovich

Publication

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

pTEF2

Expression

Yeast

Mutation

WT

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMY.005

Plasmid

#228684

Purpose

pTEF1 Promoter for yeast expression with Nterminal tag

Depositor

Chen Davidovich

Publication

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

pTEF1

Expression

Yeast

Mutation

WT

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMY.004

Plasmid

#228683

Purpose

pHHF2 Promoter for yeast expression with Nterminal tag

Depositor

Chen Davidovich

Publication

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

pHHF2

Expression

Yeast

Mutation

WT

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMY.003

Plasmid

#228682

Purpose

pPGK1 Promoter for yeast expression with Nterminal tag

Depositor

Chen Davidovich

Publication

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

pPGK1

Expression

Yeast

Mutation

WT

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMY.001

Plasmid

#228680

Purpose

pTDH3 Promoter for yeast expression with Nterminal tag

Depositor

Chen Davidovich

Publication

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

pTDH3

Expression

Yeast

Mutation

WT

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13239

Plasmid

#219912

Purpose

The base plasmid of TUNEYALI for TF49

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49

Expression

Yeast

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2-TLCV2

Plasmid

#196331

Purpose

the TLCV2 plasmid with Tol2 recombination sites for integration

Depositor

Itamar Harel

Publication

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

Cas9-2A-eGFP

Use

CRISPR; Fish expression

Expression

Bacterial, Mammalian, and Y…

Promoter

Tight TRE promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC-His+Strep-Opa1 (SB254)

Plasmid

#227603

Purpose

Inducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Inserts

SLC25A46 (SLC25A46 Human)
Opa1

Tags

10xHis and Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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