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ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

Plasmid

#207054

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

L55W T56A V61W R65A

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8-MET15

Plasmid

#207055

Purpose

Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Promoter

pATG8

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-V5-APEX2-Ura

Plasmid

#207061

Purpose

Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

V5-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-GFP-APEX2-Ura

Plasmid

#207062

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-Snf7-4V5-APEX2-URA

Plasmid

#207065

Purpose

Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pERG6-Pex3-4V5-APEX2-URA

Plasmid

#207068

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pLSP1-Pex3-4V5-APEX2-URA

Plasmid

#207069

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pLSP1

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv

Plasmid

#183733

Purpose

Expresses anti-lysozyme scFv antibody

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv_V50A

Plasmid

#183734

Purpose

Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Mutation

V50A mutation in MFalpha signal

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB6637

Plasmid

#212696

Purpose

gRNA targeting to the intergradtion site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB13089

Plasmid

#212676

Purpose

TEF promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

TEF

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12706

Plasmid

#212677

Purpose

EXP promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

EXP

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12708

Plasmid

#212678

Purpose

GPD promoter

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

GPD

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB13090

Plasmid

#212679

Purpose

Lip2 terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

Lip2

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12710

Plasmid

#212680

Purpose

Syn8 terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

Syn8

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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PCfB12709

Plasmid

#212681

Purpose

SynGuo terminator

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

SynGuo

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB8860

Plasmid

#212697

Purpose

gRNA targeting to the intergradtion site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB8856

Plasmid

#212701

Purpose

gRNA targeting to the intergradtion site C3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site C3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB8866

Plasmid

#212703

Purpose

gRNA targeting to the intergradtion site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site F3

Expression

Yeast

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB13014

Plasmid

#212685

Purpose

integration vector for single cassette with integration site E3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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