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pRH3155

Plasmid

#210175

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ade1-L32R-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::ade1-L32R-GFP::tADH1 (ADE2 Budding Yeast)

Expression

Yeast

Mutation

L32R

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3218

Plasmid

#210186

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151N-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151N-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151N

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3175

Plasmid

#210177

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

R33G,G141S

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3152

Plasmid

#210172

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ade1-G54E-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::ade1-G54E-GFP::tADH1 (ADE2 Budding Yeast)

Expression

Yeast

Mutation

G54E

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3069

Plasmid

#210163

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151V-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151V-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151V

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3047

Plasmid

#210157

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151H-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151H-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151H

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3055

Plasmid

#210161

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151Q-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151Q-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151Q

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3048

Plasmid

#210158

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151Y-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151Y-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151Y

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3053

Plasmid

#210159

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::lys1-W151A-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::lys1-W151A-GFP::tADH1 (LYS1 Budding Yeast)

Expression

Yeast

Mutation

W151A

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

Plasmid

#195048

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Expression

Yeast

Promoter

pADH2, pGPD, and pXYL

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

Plasmid

#195047

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Expression

Yeast

Promoter

pADH2, pGPD, and pXYL

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

Plasmid

#195046

Purpose

cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Inserts

crtE
crtI
crtYB

Expression

Yeast

Promoter

pADH2, pGPD, and pXYL

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1_sfGFP

Plasmid

#207801

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Promoter

TEF

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX143

Plasmid

#202816

Purpose

Control plasmid expressing mScarlet-I fluorescent protein.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

mScarlet-I

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX173

Plasmid

#202817

Purpose

Plasmid expressing the TEV protease, codon-optimized for Saccharomyces cerevisiae.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

TEV protease

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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YP46

Plasmid

#199987

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 46

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Expression

Yeast

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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186_pETcon_SARS2_XBB1-5

Plasmid

#208086

Purpose

yeast surface display of the SARS-CoV-2 XBB.1.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 XBB.1.5 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWR24

Plasmid

#202771

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coNAT
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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