8,411 results

Showing: 5501 - 5520 of 8411 results

Results per page:

Sort results by:

pSCL390

Plasmid

#219500

Purpose

Integrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpression

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Integrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT

Tags

SV40-NLS

Expression

Yeast

Promoter

GAL1-GAL10

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL391

Plasmid

#219501

Purpose

Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1

Expression

Yeast

Promoter

GAL7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL1-100

Plasmid

#219408

Purpose

Reporter gene (4 operators) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x4-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL1-422

Plasmid

#219410

Purpose

Reporter gene (1 operator) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x1-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL1-424

Plasmid

#219412

Purpose

Reporter gene (3 operator) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x3-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL1-095

Plasmid

#219407

Purpose

Reporter gene (4 operators) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(13-6 op)x4-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL1-423

Plasmid

#219411

Purpose

Reporter gene (2 operator) for zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

(42-10 op)x2-minCyc1-Venus-tCyc1

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL2-138

Plasmid

#219413

Purpose

Estradiol-inducible expression of 42-10 Zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}

Expression

Yeast

Mutation

Four Arg to Ala mutations in zinc finger backbone

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL2-160

Plasmid

#219414

Purpose

Estradiol-inducible expression of 13-6 Zinc finger activator

Depositor

Ahmad Khalil

Article

Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

Insert

{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-WT)-PDZLigWT-tSSA1}

Expression

Yeast

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRHD-TetR-GAL80-Rho1-GAL4

Plasmid

#218274

Purpose

Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

Depositor

Christopher Howard

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2

Expression

Yeast

Mutation

DHFR (P66L), Rho1 (C17R)

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM040_P8b_5'Ura4

Plasmid

#216461

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'ura4

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM036_P7_3"Ura4

Plasmid

#216465

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''ura4

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM024_P2_pMPA1

Plasmid

#216449

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter mpa1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM022_P2_pRPS1002

Plasmid

#216447

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rps1002

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM020_P2_pTRS1

Plasmid

#216445

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter trs1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416GAL-mRuby3

Plasmid

#166141

Purpose

mRuby3 expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Expression

Yeast

Promoter

GAL1

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG426GAL-ccdB-mRuby3

Plasmid

#166142

Purpose

Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

mRuby3

Expression

Yeast

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415GPD-EGFP

Plasmid

#166137

Purpose

EGFP expression under the control of the GPD promoter yeast

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

EGFP

Expression

Yeast

Promoter

GDP

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415GPD-mRuby3

Plasmid

#166138

Purpose

mRuby3 expression under control of the GPD promoter in yeast

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Expression

Yeast

Promoter

GDP

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFPEasy9R

Plasmid

#218291

Purpose

Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

Depositor

Claudia Vickers

Article

McDonnell et al bioRxiv 2024.03.26.586389

Insert

pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge