8,388 results
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Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D94 (pAF10C6)
Plasmid#185848PurposeExpressing artificial trans-repressor in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>CYC8>PABF1
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT6EG7mlh
Plasmid#185887PurposeIn vivo gene amplification (HapAmp) of limonene synthetic module at RPL25 locus (23 copy).DepositorInsertsee comments
ExpressionYeastMutationERG20 F96W N127WAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIPKAH
Plasmid#185869PurposeKnocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)DepositorInsertGPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2HMGr
Plasmid#185867PurposeKnocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiaeDepositorInsertALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2E
Plasmid#185866PurposeKnocking out ALD6DepositorInsertALD(-125, 40)- ALD6(1054, 1749)
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCble
Plasmid#185861PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF4E9D
Plasmid#185844PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>LacI>TUP1>PABF1
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9C3
Plasmid#185843PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>RevTetR_r1.7>SIN3C>PABF1
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D91
Plasmid#185845PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>SIN3C>PABF1
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT7A6
Plasmid#185896PurposeIn vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILAC2
Plasmid#185889PurposeIn vivo gene amplification (HapAmp) of lycopene synthetic module at RPL25 locusDepositorInsertsee comments
ExpressionYeastMutationERG20 F96C; Xd.CRtYB E83KAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIT6EG7m
Plasmid#185885PurposeA backbone plasmid for in vivo gene amplification (HapAmp) of a monoterpene synthetic module (+ GPP synthase without a monoterpene synthase) at RPL25 locus (14 copy)DepositorTypeEmpty backboneExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1DFB
Plasmid#185871PurposeA parental plasmid for in vivo gene amplification at RPL25 locusDepositorTypeEmpty backboneExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2S
Plasmid#185865PurposeKnocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiaeDepositorInsertALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCKan
Plasmid#185862PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF71
Plasmid#185860PurposeTesting the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B11)
Plasmid#185859PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B9)
Plasmid#185858PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72
Plasmid#185857PurposeTesting the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only