8,060 results

1. pPEE43

   Plasmid

   #139422

   Purpose

   Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mMaroon1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAM1674

   Plasmid

   #159726

   Purpose

   Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   mDHFR (Dhfr Mouse)
   

   Use

   Tags

   Gal4DBD and MPTLV-GGGGGG

   Expression

   Yeast

   Mutation

   remove start and stop codon of Dhfr

   Promoter

   ADH1

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAM1479a3

   Plasmid

   #159725

   Purpose

   Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   mDHFR (Dhfr Mouse)
   

   Use

   Tags

   Gal4DBD and MYITV-GGGGGG

   Expression

   Yeast

   Mutation

   remove start and stop codon of Dhfr

   Promoter

   ADH1

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCSJ182

   Plasmid

   #159723

   Purpose

   Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

   Depositor

   Alexander Varshavsky

   Article

   Chen et al Science. 2017 Jan 27;355(6323). pii: 355

   Insert

   GID4 (VID24 Budding Yeast)
   

   Use

   Yeast two-hybrid assay

   Tags

   3xflag and Gal4AD

   Expression

   Yeast

   Mutation

   remove stop codon

   Promoter

   ADH1

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCSJ392

   Plasmid

   #159722

   Purpose

   Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   GID10 (GID10 Budding Yeast)
   

   Use

   Yeast two-hybrid assay

   Tags

   3xflag and Gal4AD

   Expression

   Yeast

   Mutation

   remove stop codon

   Promoter

   ADH1

   Available since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPICZ-His6-K2P4.1A-mCherry-StreptagII

   Plasmid

   #158743

   Purpose

   Expresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.

   Depositor

   Arthur Laganowsky

   Article

   Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

   Insert

   K2P4.1-A (KCNK4 Human)
   

   Use

   Tags

   His6 and mCherry with Streptag II

   Expression

   Yeast

   Mutation

   Residues 1-264 with N-linked glycosylation sites …

   Promoter

   AOX1

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPICZ-His6-K2P4.1B-mCherry-StreptagII

   Plasmid

   #158744

   Purpose

   Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

   Depositor

   Arthur Laganowsky

   Article

   Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

   Insert

   K2P4.1-B (KCNK4 Human)
   

   Use

   Tags

   His6 and mCherry with Streptag II

   Expression

   Yeast

   Mutation

   Residues 1-290 and N-linked glycosylation sites r…

   Promoter

   AOX1

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p415 Glo3 S389,398 A

   Plasmid

   #129485

   Purpose

   Expression in S. cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 S389,398 A (GLO3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Mutation of serines 389 and 398 to alanine

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p415 Glo3 S389,398 D

   Plasmid

   #129486

   Purpose

   Expression in S. cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 S389,398 D (GLO3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Mutation of serines 389 and 398 to Asp

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p415 Met25 Glo3 ΔGRM-ΔC

    Plasmid

    #129487

    Purpose

    Expression in S. cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 ΔGRM ΔC (GLO3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Δ376-493 (deletion of Glo3 motif & C-terminal…

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p415 Glo3 R59K

    Plasmid

    #112652

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 R59K
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R59K (GAP-dead)

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p415 Glo3 R59K ΔC

    Plasmid

    #112653

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 R59K ΔC
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R59K, Δ431-493 (deletion of C-terminal amphipathi…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p415 Glo3 R59K ΔGRM-ΔC

    Plasmid

    #112654

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 R59K ΔGRM ΔC
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R59K, Δ376-493 (deletion of Glo3 motif & C-te…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p415 Glo3 R59K Δ2x+ve

    Plasmid

    #112655

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 R59K Δ2x+ve
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R59K, mutations (R223E, K224E, K225E, K233E, K234…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p415 Glo3 R59K S389,398 A

    Plasmid

    #112656

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 R59K S389,398A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R59K, mutations (S389A, S398A) in Glo3 motif, non…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p415 Glo3 R59K S389,398 D

    Plasmid

    #112657

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 R59K S389,398D
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R59K, mutations (S389D, S398D) in Glo3 motif, pho…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p415 GFP-Glo3 ΔGRM-ΔC

    Plasmid

    #112659

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP-Glo3 ΔGRM ΔC
    

    Use

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Δ376-493 (deletion of Glo3 motif & C-terminal…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p415 GFP-Glo3 S389,398 A

    Plasmid

    #112660

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP- Glo3 S389,398A
    

    Use

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    mutations (S389A, S398A) in Glo3 motif, non-phosp…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p415 GFP-Glo3 S389,398 D

    Plasmid

    #112661

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP-Glo3 S389,398D
    

    Use

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    mutations (S389D, S398D) in Glo3 motif, phosphomi…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p415 GFP-Glo3 Δ2x+ve

    Plasmid

    #112662

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP- Glo3 Δ2x+ve
    

    Use

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Mutations (R223E, K224E, K225E, K233E, K234E, K23…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge