8,126 results
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Plasmid#164708PurposeyEVenus under the control of tetOpr with ADH1t upstream of the reporterDepositorInsertADH1t - tetOpr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG88
Plasmid#164709PurposeyEVenus under the control of ARG3 promoterDepositorInsertARG3pr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-KAR2-mEGFP-HDEL
Plasmid#160467PurposeYeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signalDepositorInsertKAR2-mEGFP-HDEL
ExpressionYeastAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL10
Plasmid#164710PurposeyEVenus under the control of MET3 promoterDepositorInsertMET3pr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-PpSEC13-mEGFP
Plasmid#160469PurposeP. pastoris integration vector for the gene replacement of SEC13 fused to mEGFPDepositorInsertppSEC13-mEGFP
ExpressionYeastAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-PpSEC13-msGFP2
Plasmid#160470PurposeP. pastoris integration vector for the gene replacement of SEC13 fused to msGFP2DepositorInsertppSEC13-msGFP2
ExpressionYeastAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 ΔN
Plasmid#163575Purposeexpress wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
TagsGFPExpressionYeastMutationTruncation of N-terminal domain (1-242) of Dcp2PromoterDCP2 promoterAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 300
Plasmid#163577Purposeexpress Dcp2 300 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
TagsGFPExpressionYeastMutationTruncation of C-terminal domian (301-970)PromoterDCP2 promoterAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI763 p(Z)-mCherry + UBC9 sgRNA
Plasmid#164918PurposeCEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNADepositorInsertP(Z)-mcherry
UseCRISPRExpressionYeastPromoterP(Z), synthetic promoterAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)
Plasmid#164916PurposeExpresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTRDepositorInsertGCN4(UTR)-ZEM(TF)
UseSynthetic BiologyExpressionYeastPromoterPGK1 (yeast)Available SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI743 dual-barcoded gRNA parent vector
Plasmid#164915PurposeCEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid librariesDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI733 pRPR1(TetO)-RPC31-sgRNA
Plasmid#164913PurposeFor yeast genomic integration of sgRNA against RPC31DepositorInsertpRPR1(TetO)-RPC31-sgRNA
UseCRISPRExpressionYeastPromoterRPR1Available SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI732 pRPR1(TetO)-HTS1-sgRNA
Plasmid#164912PurposeFor yeast genomic integration of sgRNA against HTS1DepositorInsertpRPR1(TetO)-HTS1-sgRNA
UseCRISPRExpressionYeastPromoterRPR1Available SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEE42
Plasmid#139421PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.DepositorInsertCitrine
ExpressionYeastPromoterTEF1Available SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS423-ProSgH
Plasmid#163974PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoterDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPAP003
Plasmid#153490PurposeLevel 2 integrative plasmid for stable expression in P. pastorisDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20F
Plasmid#145789PurposemPing Yeast Transposition AssayDepositorInsertmmPing20F
ExpressionYeastMutationInsertion of the FMV enhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20B
Plasmid#145790PurposemPing Yeast Transposition AssayDepositorInsertmmPing20B
ExpressionYeastMutationInsertion of the b-Conglycin EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A NL60
Plasmid#145791PurposemPing Yeast Transposition AssayDepositorInsertNL60
ExpressionYeastMutationInsertion of the OCS EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A 2xE
Plasmid#145792PurposemPing Yeast Transposition AssayDepositorInsert2xE
ExpressionYeastMutationInsertion of 2 copies of the CMV EnhancerAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only