8,126 results
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Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyExpressionYeastPromoteract1Available SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL27-decoder
Plasmid#159449PurposeInsert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPfus1tet-citrine-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1tetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1tetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVD_01
Plasmid#149606PurposepTEF1-VanR inserted in pRS416 backboneDepositorInsertVanR
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJPS2957
Plasmid#135591PurposeSNR6 has GAGAUUUAUUUC at position 96–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
ExpressionYeastMutationU6Δ12 has GAGAUUUAUUUC at position 96–107 deletedPromoterNativeAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG569
Plasmid#158588PurposetNCS-ePTS1 Cen6/ARS4DepositorInserttNCS-ePTS1
ExpressionYeastAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJB1-RPL1B-Cl
Plasmid#160430PurposeExpresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoterDepositorInsertRPL1B
TagsmCherryExpressionYeastMutationRemoved introns, shuffled codons to vary nucleoti…PromoterS. cerevisiae GAL1Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD217
Plasmid#160939PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mTagBFP2-HA Tag
TagsHA tagExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD118
Plasmid#160938PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mCherry-FLAG tag
TagsMYC tagExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD115
Plasmid#160937PurposeExcess Capsid Protein for in vivo mRNA displayDepositorInsertMCP-mCherry-MYC tag
TagsMYC tagExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD163
Plasmid#160935PurposeIn vivo mRNA display construct for mCherryDepositorInsertMCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
TagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1160c2
Plasmid#159724PurposeExpress MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
TagsGal4DBD and MPGIL-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only