8,126 results

1. pPICZ-aC-VTS-US

   Plasmid

   #160425

   Purpose

   Expression of c-luciferase from sp. Vargula tsujii (USA)

   Depositor

   Todd Oakley

   Article

   Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   6x Histidine tag, Yeast alpha secretion factor, c…

   Expression

   Yeast

   Mutation

   See depositor comments below

   Promoter

   AOX

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPICZ-aC-MSVU-BZ

   Plasmid

   #160426

   Purpose

   Expression of c-luciferase from sp. Maristella sp. SVU (Belize)

   Depositor

   Todd Oakley

   Article

   Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   6x Histidine tag, Yeast alpha secretion factor, c…

   Expression

   Yeast

   Mutation

   See depositor comments below

   Promoter

   AOX

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPSG1059

   Plasmid

   #158595

   Purpose

   tNCS-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4)

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Inserts

   tNCS-ePTS1
   Ec_CYP80B1
   Ps_6OMT
   Ps_4'OMT2
   Ps_CNMT
   

   Expression

   Yeast

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPSG1060

   Plasmid

   #158596

   Purpose

   tNCS-dead-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4)

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Inserts

   tNCS-dead_ePTS1
   Ec_CYP80B1
   Ps_6OMT
   Ps_4'OMT2
   Ps_CNMT
   

   Expression

   Yeast

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPSG1067

   Plasmid

   #158597

   Purpose

   tNCS-Venus (CEN6/ARS4)

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Insert

   tNCS-Venus-ePTS1
   

   Expression

   Yeast

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJJB207

   Plasmid

   #158599

   Purpose

   UbiY-Venus-ePTS1

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Insert

   UbiY-Venus-ePTS1
   

   Tags

   Ubi-Y and ePTS1

   Expression

   Yeast

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPSG1066

   Plasmid

   #158590

   Purpose

   tNCS-Venus-ePTS1 CEN6/ARS4

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Insert

   tNCS-Venus-ePTS1
   

   Expression

   Yeast

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJAS1643

   Plasmid

   #158591

   Purpose

   tNCS-VioE CEN6/ARS4

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Insert

   tNCS-VioE-ePTS1
   

   Expression

   Yeast

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJAS1644

   Plasmid

   #158592

   Purpose

   tNCS-VioE-dead_ePTS1 CEN6/ARS4

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Insert

   tNCS-VioE-dead_ePTS1
   

   Expression

   Yeast

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDUAL-Pnmt1-Ost4-GFP

    Plasmid

    #154368

    Purpose

    Yeast expression of Ost4

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Ost4 (ost4 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPEE45

    Plasmid

    #139424

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mTurquoise1
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPEE44

    Plasmid

    #139423

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mTFP1
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPEE43

    Plasmid

    #139422

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mMaroon1
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAM1674

    Plasmid

    #159726

    Purpose

    Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    mDHFR (Dhfr Mouse)
    

    Tags

    Gal4DBD and MPTLV-GGGGGG

    Expression

    Yeast

    Mutation

    remove start and stop codon of Dhfr

    Promoter

    ADH1

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAM1479a3

    Plasmid

    #159725

    Purpose

    Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    mDHFR (Dhfr Mouse)
    

    Tags

    Gal4DBD and MYITV-GGGGGG

    Expression

    Yeast

    Mutation

    remove start and stop codon of Dhfr

    Promoter

    ADH1

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCSJ182

    Plasmid

    #159723

    Purpose

    Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

    Depositor

    Alexander Varshavsky

    Article

    Chen et al Science. 2017 Jan 27;355(6323). pii: 355

    Insert

    GID4 (VID24 Budding Yeast)
    

    Use

    Yeast two-hybrid assay

    Tags

    3xflag and Gal4AD

    Expression

    Yeast

    Mutation

    remove stop codon

    Promoter

    ADH1

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSJ392

    Plasmid

    #159722

    Purpose

    Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    GID10 (GID10 Budding Yeast)
    

    Use

    Yeast two-hybrid assay

    Tags

    3xflag and Gal4AD

    Expression

    Yeast

    Mutation

    remove stop codon

    Promoter

    ADH1

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPICZ-His6-K2P4.1A-mCherry-StreptagII

    Plasmid

    #158743

    Purpose

    Expresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.

    Depositor

    Arthur Laganowsky

    Article

    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

    Insert

    K2P4.1-A (KCNK4 Human)
    

    Tags

    His6 and mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Residues 1-264 with N-linked glycosylation sites …

    Promoter

    AOX1

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPICZ-His6-K2P4.1B-mCherry-StreptagII

    Plasmid

    #158744

    Purpose

    Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

    Depositor

    Arthur Laganowsky

    Article

    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

    Insert

    K2P4.1-B (KCNK4 Human)
    

    Tags

    His6 and mCherry with Streptag II

    Expression

    Yeast

    Mutation

    Residues 1-290 and N-linked glycosylation sites r…

    Promoter

    AOX1

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p415 Glo3 S389,398 A

    Plasmid

    #129485

    Purpose

    Expression in S. cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 S389,398 A (GLO3 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Mutation of serines 389 and 398 to alanine

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge