8,411 results
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Plasmid#174298PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
TagsHA and cMycExpressionYeastMutationN343Q, E484KAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#174070PurposeExpresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGal-Olac-3xNLS-VP16-CIB1-tADH1
TagsFLAGExpressionYeastPromoterpGal1Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL29
Plasmid#174063PurposeExpresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.DepositorInsert2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
TagsFLAGExpressionYeastPromoterpCyc1Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL15
Plasmid#174069PurposeExpresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
TagsFLAGExpressionYeastPromoterpGal1Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)
Plasmid#173462PurposeC-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)
Plasmid#173460PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen (pJJF003)
Plasmid#173459PurposeC-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)
Plasmid#173458PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4953
Plasmid#174020PurposepDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoterDepositorInsertAtg6
TagsGFP, Atg6ExpressionYeastPromoter41nmt1Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4658
Plasmid#174017PurposepDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoterDepositorInsertAtg8
TagsGFP, Atg8ExpressionYeastPromoter41nmt1Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4958
Plasmid#174014PurposepHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoterDepositorInsertAtg6
TagsCFP, Atg6ExpressionYeastPromoter41nmt1Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4924
Plasmid#174012PurposepDUAL plasmid expressing Pil1-mCherry from 41nmt1 promoterDepositorInsertPil1-mCherry
TagsPil1, mCherryExpressionYeastPromoter41nmt1 and 41nmt1pAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4948
Plasmid#174019PurposepDUAL plasmid expressing Pil1-mCherry-Vps38 from 41nmt1 promoterDepositorInsertVps38
TagsPil1, mCherry, Vps38ExpressionYeastPromoter41nmt1Available SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4946
Plasmid#174018PurposepDUAL plasmid expressing Pil1-mCherry-Atg14 from 41nmt1 promoterDepositorAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4925
Plasmid#174016PurposepDUAL plasmid expressing Pil1-mCherry-Atg38(161-190) from 41nmt1 promoterDepositorInsertAtg38(161-190)
TagsPil1, mCherry, Atg38(161-190)ExpressionYeastPromoter41nmt1Available SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS697
Plasmid#168778PurposeYeast surface display plasmid backbone that can be combined with pJS699 through homologous recombinationDepositorTypeEmpty backboneTagsCmyc and HAExpressionYeastAvailable SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6-U57A
Plasmid#169927PurposeWT U6 with a U57A mutationDepositorInsertU6 U57A
ExpressionBacterial and YeastAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 Nxf1B
Plasmid#170266PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing full length Nxf1BDepositorArticleInsertnuclear export factor 1, B6 allele
TagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 Nxf1C
Plasmid#170267PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing full length Nxf1CDepositorArticleInsertnuclear export factor 1, CAST allele
TagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 mCherry
Plasmid#170264PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mCherryDepositorArticleInsertmomeric Cherry
TagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only