8,060 results
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Plasmid#79020PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only
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pREP42xV5_ccdB
Plasmid#79018PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA186
Plasmid#74250PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).DepositorInsertInducible peptide of the dPSTR
UseTagsExpressionBacterial and YeastMutationPromoterpGPD1Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA171
Plasmid#74251PurposeIntermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
UseTagsVenusExpressionBacterial and YeastMutationPromoterpSTL1Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA199
Plasmid#74275PurposepSTL1-dPSTRy(4,3)DepositorInsertpSTL1-dPSTRy(4,3)
UseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4-Bre1
Plasmid#73617PurposeControl of the E3 ligase Bre1 with LINXa in yeast.DepositorInsertLINXa4-Bre1dNLS
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK139
Plasmid#73315PurposePas2p peroxisomal membrane biogenesis proteinDepositorInsertPas2p peroxisomal membrane targeting protein
UseTagsHis6 and c-mycExpressionYeastMutationEncodes a glycine-204 to serine mutationPromoterAOX1Available SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
Plasmid#68276PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLSDepositorInsertSte7DS NLS(4E) mCherry
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2336
Plasmid#67541PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK275
Plasmid#73363PurposeEncodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)DepositorInsertdelta-8 sphingolipid desaturase
UseTagsExpressionYeastMutationmissing residues 1-6PromoterGAL1Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK138
Plasmid#73314PurposePas2p peroxisomal membrane targeting sequenceDepositorInsertPas2p (peroxisomal membrane targeting region)
UseTagsHis6 and c-mycExpressionYeastMutationencodes residues 1-42PromoterAOX1Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK141
Plasmid#73316PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6, Pas2p signal sequence, and c-mycExpressionYeastMutationsynthetic genePromoterAOX1Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK137
Plasmid#73313PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6 and c-mycExpressionYeastMutationsynthetic genePromoter5'-AOX1Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD125 GFP:POP1
Plasmid#71387Purposelocalization of yeast RNase POP1 (RNase MRP binding protein) in yeastDepositorAvailable SinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUG35-YDL183c-GFP
Plasmid#70161Purposeyeast expression of YDL183c with GFP fusionDepositorInsertYDL183c
UseTagsyEGFPExpressionYeastMutationPromoterMET17Available SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
YCp33-YDL183c-HA
Plasmid#70164Purposeyeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal endDepositorInsertYDL183c
UseTags3xHAExpressionYeastMutationPromoterYDL183c (217 nucleotides upstream of the ATG)Available SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK349
Plasmid#71566PurposeProduces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeastDepositorInsertdelta12 fatty acid acetylenase
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS2-aurZ
Plasmid#70151PurposeFusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection markerDepositorInsertaurZ
UseTagsExpressionYeastMutationPromoterTefAvailable SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMES140
Plasmid#71849PurposeComplementation of NME1 gene disruption or mutation in yeastDepositorAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK274
Plasmid#71564PurposeProduces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeastDepositorInsertdelta12 fatty acid desaturase (form 3)
UseTagsExpressionBacterial and YeastMutationPromoterGAL1 promoterAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only