8,126 results

1. pWHI2-HYG

   Plasmid

   #80589

   Purpose

   CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

   Depositor

   John McCusker

   Article

   Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

   Insert

   WHI2 (WHI2 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   WHI2 native promoter

   Available Since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pFA6a-pcr1P-natMX6-pcr2P

   Plasmid

   #80620

   Purpose

   to delete part of the mat1-P locus and fix the mating type in natural S. pombe isolates

   Depositor

   Miguel Godinho Ferreira

   Article

   Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

   Inserts

   PCR1 from mat1-P
   PCR2 from mat1-P
   

   Expression

   Yeast

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-pcr1M-natMX6-pcr2M

   Plasmid

   #80621

   Purpose

   to delete part of the mat1-M locus and fix the mating type in natural S. pombe isolates

   Depositor

   Miguel Godinho Ferreira

   Article

   Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

   Inserts

   PCR1 from mat1-M
   PCR2 from mat1-M
   

   Expression

   Yeast

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pREP81xV5_ccdB

   Plasmid

   #79019

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction

   Depositor

   Göran Akusjärvi, Pernilla Bjerling

   Type

   Empty backbone

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pREP41xV5_ccdB

   Plasmid

   #79017

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection

   Depositor

   Göran Akusjärvi, Pernilla Bjerling

   Type

   Empty backbone

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pREP42xN_ccdB

   Plasmid

   #79024

   Purpose

   Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection

   Depositor

   Göran Akusjärvi, Pernilla Bjerling

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pREP82xV5_ccdB

   Plasmid

   #79020

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection

   Depositor

   Göran Akusjärvi, Pernilla Bjerling

   Type

   Empty backbone

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pREP42xV5_ccdB

   Plasmid

   #79018

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection

   Depositor

   Göran Akusjärvi, Pernilla Bjerling

   Type

   Empty backbone

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDA186

   Plasmid

   #74250

   Purpose

   Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).

   Depositor

   Serge Pelet

   Article

   Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

   Insert

   Inducible peptide of the dPSTR
   

   Expression

   Bacterial and Yeast

   Promoter

   pGPD1

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDA171

    Plasmid

    #74251

    Purpose

    Intermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    Inducible peptide of the dPSTR
    

    Tags

    Venus

    Expression

    Bacterial and Yeast

    Promoter

    pSTL1

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDA199

    Plasmid

    #74275

    Purpose

    pSTL1-dPSTRy(4,3)

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    pSTL1-dPSTRy(4,3)
    

    Expression

    Bacterial and Yeast

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNIA-CEN-FLAG-LINXa4-Bre1

    Plasmid

    #73617

    Purpose

    Control of the E3 ligase Bre1 with LINXa in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa4-Bre1dNLS
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK139

    Plasmid

    #73315

    Purpose

    Pas2p peroxisomal membrane biogenesis protein

    Depositor

    T. J. Kappock

    Insert

    Pas2p peroxisomal membrane targeting protein
    

    Tags

    His6 and c-myc

    Expression

    Yeast

    Mutation

    Encodes a glycine-204 to serine mutation

    Promoter

    AOX1

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry

    Plasmid

    #68276

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS(4E) mCherry
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    RPS2

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB2336

    Plasmid

    #67541

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK275

    Plasmid

    #73363

    Purpose

    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)

    Depositor

    T. J. Kappock

    Insert

    delta-8 sphingolipid desaturase
    

    Expression

    Yeast

    Mutation

    missing residues 1-6

    Promoter

    GAL1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK138

    Plasmid

    #73314

    Purpose

    Pas2p peroxisomal membrane targeting sequence

    Depositor

    T. J. Kappock

    Insert

    Pas2p (peroxisomal membrane targeting region)
    

    Tags

    His6 and c-myc

    Expression

    Yeast

    Mutation

    encodes residues 1-42

    Promoter

    AOX1

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK141

    Plasmid

    #73316

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Tags

    His6, Pas2p signal sequence, and c-myc

    Expression

    Yeast

    Mutation

    synthetic gene

    Promoter

    AOX1

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK137

    Plasmid

    #73313

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tag

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Tags

    His6 and c-myc

    Expression

    Yeast

    Mutation

    synthetic gene

    Promoter

    5'-AOX1

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTD125 GFP:POP1

    Plasmid

    #71387

    Purpose

    localization of yeast RNase POP1 (RNase MRP binding protein) in yeast

    Depositor

    Mark Schmitt

    Article

    Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

    Insert

    POP1 (POP1 Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    ACT1

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only