8,060 results

1. pMZ286

   Plasmid

   #52227

   Purpose

   Expression of sgRNA precursor targeted against ade6L469

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Insert

   rrk1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   rrk1

   Available Since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. p41Nat 3-F GW

   Plasmid

   #58553

   Purpose

   CEN vector with clonNat resistance marker for triple Gateway insertion

   Depositor

   Leonid Kruglyak

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p416GAL1-Skn7t-L-IFP-F[1] (Yeast)

   Plasmid

   #52896

   Purpose

   Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Skn7t-L-IFP-F[1]
   

   Use

   Tags

   L-IFP-F[1]

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

   Plasmid

   #52898

   Purpose

   YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Ypd*1-L-IFP-F[2]
   

   Use

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Mutation

   Changed Tryptophane 80 to Alanine

   Promoter

   GAL1

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pDL86

   Plasmid

   #43909

   Depositor

   Stephen Elledge

   Article

   Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

   Insert

   DIF1 (DIF1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pGLx2-set1-H1017K

   Plasmid

   #53258

   Purpose

   GAL driven LexA-tagged SET1-catalytically dead (H1017K)

   Depositor

   Jean Cook

   Article

   Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

   Insert

   SET1
   

   Use

   Tags

   LexA

   Expression

   Yeast

   Mutation

   H1017K mutation - catalytically dead

   Promoter

   GAL1/10

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZHY566

   Plasmid

   #50608

   Purpose

   TALEN expression vector for yeast, N152,C18 architecture

   Depositor

   Daniel Voytas

   Article

   Zhang et al Plant Physiol. 2012 Nov 2.

   Type

   Empty backbone

   Use

   TALEN

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZHY565

   Plasmid

   #50607

   Purpose

   TALEN expression vector for yeast, N152,C18 architecture

   Depositor

   Daniel Voytas

   Article

   Zhang et al Plant Physiol. 2012 Nov 2.

   Type

   Empty backbone

   Use

   TALEN

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available Since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSS63

   Plasmid

   #47951

   Purpose

   rtTA positive feedback loop with single tet operator in the GAL1 promoter

   Depositor

   Attila Becskei

   Article

   Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

   Insert

   rtTA
   

   Use

   Tet inducible expression

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   T3

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-yomEmerald-CaURA3

    Plasmid

    #44890

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mEmerald with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomEmerald
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAH4

    Plasmid

    #41828

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistance

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCM305

    Plasmid

    #44550

    Depositor

    Michael Grunstein

    Article

    Millar et al Genes Dev. 2006 Mar 15;20(6):711-22.

    Insert

    HA-htz1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pFA6a-link-PhiYellow-SpHis5

    Plasmid

    #44651

    Purpose

    Yeast tagging vector to create gene fusion with PhiYellow with SpHis5 selection

    Depositor

    Kurt Thorn

    Insert

    phiYellow
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDR-AmTrac-GS

    Plasmid

    #47769

    Purpose

    expresses AmTrac-GS in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac-GS
    

    Use

    Tags

    mcpGFP

    Expression

    Yeast

    Mutation

    Promoter

    PMA

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pDL128

    Plasmid

    #43912

    Depositor

    Stephen Elledge

    Article

    Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

    Insert

    DIF1 (DIF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    SML domain mutated PPMLINQRT->AAAAAAAA, S59G, …

    Promoter

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pDL127

    Plasmid

    #43911

    Depositor

    Stephen Elledge

    Article

    Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

    Insert

    DIF1 (DIF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HUG domain mutated VGMRIRQ->AAAAAAA, S59, Q66H

    Promoter

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pDL96

    Plasmid

    #43910

    Depositor

    Stephen Elledge

    Article

    Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

    Insert

    DIF1 (DIF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    AAEAAA

    Promoter

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pFA5

    Plasmid

    #46791

    Purpose

    To express a version of Fun30 without CUE domain in budding yeast

    Depositor

    Patrick Varga-Weisz

    Article

    Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

    Insert

    FUN30 without CUE domain (FUN30 Budding Yeast)
    

    Use

    Tags

    V5

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pFA12

    Plasmid

    #46792

    Purpose

    To express a version of Fun30 with a mutated CUE domain in budding yeast

    Depositor

    Patrick Varga-Weisz

    Article

    Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

    Insert

    FUN30, CUE domain mutated (FUN30 Budding Yeast)
    

    Use

    Tags

    V5

    Expression

    Yeast

    Mutation

    FP82AA

    Promoter

    Available Since

    Aug. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

20. UPR pBARUra3

    Plasmid

    #45354

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

    Insert

    DHFR Ub Rek Ura3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pCUP1

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only