8,126 results

1. LD120

   Plasmid

   #69171

   Purpose

   Integration of GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg20-EGFP
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pED55 pRS306 pRPS2-SteND-NLS-mCherry

   Plasmid

   #68277

   Purpose

   SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Ste7ND NLS mCherry
   

   Tags

   mCherry

   Expression

   Yeast

   Promoter

   pRPS2

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

   Plasmid

   #68275

   Purpose

   SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Ste7DS NLS(4A) mCherry
   

   Tags

   mCherry

   Expression

   Yeast

   Promoter

   pRPS2

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCyc-Cdc24

   Plasmid

   #66574

   Purpose

   Express an additional copy of Cdc24, integrated at TRP locus

   Depositor

   Orion Weiner

   Article

   Jost et al ACS Synth Biol. 2015 Jun 2.

   Insert

   Cdc24
   

   Expression

   Yeast

   Promoter

   pCyc1

   Available Since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRPR1_g2gRNA_RPR1t

   Plasmid

   #64388

   Purpose

   encodes g2 gRNA

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   g2 gRNA
   

   Expression

   Yeast

   Promoter

   pRPR1

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pRS424-TUB2 - human TUBB3 Cterminal tail delta K

   Plasmid

   #60404

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Expression

   Yeast

   Mutation

   TUB2 amino acids 429 - end, replaced with human T…

   Promoter

   GAL

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pRS424-TUB2 - human TUBB1 Cterminal tail

   Plasmid

   #60400

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Expression

   Yeast

   Mutation

   TUB2 amino acids 429 - end, replaced with human T…

   Promoter

   GAL

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pRS424-TUB2 - human TUBB3 Cterminal tail

   Plasmid

   #60397

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Expression

   Yeast

   Mutation

   C127S; TUB2 amino acids 429 - end, replaced with …

   Promoter

   GAL

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pRPR1_c2gRNA_RPR1t

   Plasmid

   #64380

   Purpose

   encodes c2 gRNA

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   c2 gRNA
   

   Expression

   Yeast

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pRPR1_g1gRNA_RPR1t

    Plasmid

    #64387

    Purpose

    encodes g1 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    g1 gRNA
    

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pRPR1_c7gRNA_RPR1t

    Plasmid

    #64385

    Purpose

    encodes c7 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c7 gRNA
    

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pRPR1_c1gRNA_RPR1t

    Plasmid

    #64379

    Purpose

    encodes c1 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c1 gRNA
    

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pRPR1_c5gRNA_RPR1t

    Plasmid

    #64383

    Purpose

    encodes c5 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c5 gRNA
    

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. Gal4pBS13-Pleum

    Plasmid

    #60332

    Purpose

    Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pJW1509

    Plasmid

    #62363

    Purpose

    pFA6a BirA-heh2 linker-SSH1::HIS5

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    BirA-heh2-Ssh1
    

    Expression

    Yeast

    Promoter

    Gal1

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGAD-hPLEKHM1

    Plasmid

    #64145

    Purpose

    For yeast two hybrid

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    PLEKHM1 (PLEKHM1 Human)
    

    Tags

    GAL4 activation domain

    Expression

    Yeast

    Promoter

    ADH

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGAD-hRubicon

    Plasmid

    #64144

    Purpose

    For yeast two hybrid

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    Rubicon (RUBCN Human)
    

    Tags

    GAL4 activation domain

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pNB786

    Plasmid

    #60151

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNB762

    Plasmid

    #60150

    Purpose

    Firefly luciferase (Promega) driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNB767

    Plasmid

    #60159

    Purpose

    Firefly luciferase (Promega) driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Expression

    Yeast

    Mutation

    A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge