8,388 results

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LD120

Plasmid

#69171

Purpose

Integration of GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg20-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pED55 pRS306 pRPS2-SteND-NLS-mCherry

Plasmid

#68277

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7ND NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

Plasmid

#68275

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS(4A) mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCyc-Cdc24

Plasmid

#66574

Purpose

Express an additional copy of Cdc24, integrated at TRP locus

Depositor

Orion Weiner

Article

Jost et al ACS Synth Biol. 2015 Jun 2.

Insert

Cdc24

Expression

Yeast

Promoter

pCyc1

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pRPR1_g2gRNA_RPR1t

Plasmid

#64388

Purpose

encodes g2 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

g2 gRNA

Expression

Yeast

Promoter

pRPR1

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB3 Cterminal tail delta K

Plasmid

#60404

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB1 Cterminal tail

Plasmid

#60400

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB3 Cterminal tail

Plasmid

#60397

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

C127S; TUB2 amino acids 429 - end, replaced with …

Promoter

GAL

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c2gRNA_RPR1t

Plasmid

#64380

Purpose

encodes c2 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c2 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_g1gRNA_RPR1t

Plasmid

#64387

Purpose

encodes g1 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

g1 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c7gRNA_RPR1t

Plasmid

#64385

Purpose

encodes c7 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c7 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c1gRNA_RPR1t

Plasmid

#64379

Purpose

encodes c1 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c1 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c5gRNA_RPR1t

Plasmid

#64383

Purpose

encodes c5 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c5 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
Gal4pBS13-Pleum

Plasmid

#60332

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

May 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pJW1509

Plasmid

#62363

Purpose

pFA6a BirA-heh2 linker-SSH1::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-heh2-Ssh1

Expression

Yeast

Promoter

Gal1

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGAD-hPLEKHM1

Plasmid

#64145

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

PLEKHM1 (PLEKHM1 Human)

Tags

GAL4 activation domain

Expression

Yeast

Promoter

ADH

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pGAD-hRubicon

Plasmid

#64144

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

Rubicon (RUBCN Human)

Tags

GAL4 activation domain

Expression

Yeast

Promoter

ADH1

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pNB786

Plasmid

#60151

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LEU1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB762

Plasmid

#60150

Purpose

Firefly luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LEU1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB767

Plasmid

#60159

Purpose

Firefly luciferase (Promega) driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

SIC1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge