8,060 results

1. pDSM-f3-Ptdh3-ACDH-Trpl3

   Plasmid

   #127746

   Purpose

   Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   acetylating acetaldehyde dehydrogenase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Ptdh3

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEMY01CD-TER

   Plasmid

   #127751

   Purpose

   Integrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAW

   Plasmid

   #133893

   Purpose

   Expression of Gal4AD-Prey hybrid protein

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBW

   Plasmid

   #133897

   Purpose

   Expression of Gal4BD-Bait hybrid protein.

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBSWHhc-Lam

   Plasmid

   #133902

   Purpose

   Negative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   LMNA (Lam) (LMNA Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   T7

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMS22H-IRE

   Plasmid

   #133905

   Purpose

   Positive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   IRE (Ftl1 Rat)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pol III

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHCMM1

   Plasmid

   #167452

   Purpose

   Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

   Depositor

   Hugo Cesar Medina-Munoz, Marvin Wickens

   Article

   Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHCMM2

   Plasmid

   #167453

   Purpose

   Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

   Depositor

   Hugo Cesar Medina-Munoz, Marvin Wickens

   Article

   Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PSEC63

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHCMM3

   Plasmid

   #167454

   Purpose

   A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

   Depositor

   Hugo Cesar Medina-Munoz, Marvin Wickens

   Article

   Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   PSEC63_yeGFP_cePUP-2_URA3_TADH1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PSEC63

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p414GPD-3xFLAG-Nop4 L306P

    Plasmid

    #169262

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306P

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 L306P (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    p.(Leu306Pro)

    Promoter

    GPD

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p414GPD-3xFLAG-Nop4 dE5

    Plasmid

    #169263

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 delta E5 (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted region corresponding to human Exon 5

    Promoter

    GPD

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p414GPD-3xFLAG-Nop4 dE8

    Plasmid

    #169264

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 delta E8 (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted region corresponding to human Exon 8

    Promoter

    GPD

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p414GPD-3xFLAG-RBM28 WT

    Plasmid

    #169265

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WT

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 WT (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p414GPD-3xFLAG-RBM28 L351P

    Plasmid

    #169266

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351P

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 L351P (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    p.(Leu351Pro)

    Promoter

    GPD

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p414GPD-3xFLAG-RBM28 dE5

    Plasmid

    #169267

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 delta E5 (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted Exon 5

    Promoter

    GPD

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p414GPD-3xFLAG-RBM28 dE8

    Plasmid

    #169268

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 delta E8 (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted Exon 8

    Promoter

    GPD

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. WT Ecm2

    Plasmid

    #169922

    Purpose

    Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ecm2 1-143

    Plasmid

    #169923

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-143
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ecm2 1-265

    Plasmid

    #169925

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-265
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ecm2 1-325

    Plasmid

    #169926

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-325
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge