We narrowed to 16,111 results for: form

1. pCHC027 (∆pemK)

   Plasmid

   #226204

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Upstream/downstream homology arms for addiction system toxinPemK.
   

   Use

   Synthetic Biology

   Available Since

   Nov. 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

   Plasmid

   #225649

   Purpose

   tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

   Depositor

   Andre Sanches Ribeiro

   Article

   Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

   Insert

   tetR/tetA, araBAD and LacO3O1 in tandem conformation
   

   Tags

   mCherry

   Expression

   Bacterial

   Promoter

   tetA, araBAD and LacO3O1 promoters

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1 WT1 +378 ATG

   Plasmid

   #217644

   Purpose

   Dox inducible WT1 cDNA - as transcribed from +378ATG (short isoform)

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 short isoform (WT1 Human)
   

   Expression

   Mammalian

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET SUMO-syt7 (136-403) L361C

   Plasmid

   #213622

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 (136-403) L361C

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 (136-403) L361C (Syt7 Mouse)
   

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   C260A C275A L361C

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET SUMO-cPLA2 C2 domain (Y96C)

   Plasmid

   #213633

   Purpose

   Bacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   cPLA2 C2 domain (Y96C) (PLA2G4A Human)
   

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Y96C, C139A, C141S

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBeast-pT7-codA

   Plasmid

   #190058

   Purpose

   Express the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   codA
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD2000

   Plasmid

   #101197

   Purpose

   Name: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.

   Depositor

   Dirk Dittmer

   Article

   Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

   Insert

   LANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
   

   Use

   Luciferase

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.2-Cx43-HA

   Plasmid

   #49851

   Purpose

   Encodes human connexin 43 with a C-terminal HA epitope fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Contains several wobble mutations to confer resis…

   Promoter

   CMV

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b Ag43 160N 6H sfGFP-R5 AmpR

   Plasmid

   #225139

   Purpose

   Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

   Depositor

   Urartu Seker

   Article

   Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

   Insert

   Ag43 protein fused to silaffin-R5 and sfGFP
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2plus-mKate2

    Plasmid

    #162624

    Purpose

    Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    mKate2
    

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUPER-retro-puro-Smad4

    Plasmid

    #89829

    Purpose

    Knockdown human Smad4 expression in human mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

    Insert

    Smad4 shRNA (SMAD4 Mouse, Human)
    

    Use

    Retroviral

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2-Cx43-siResist

    Plasmid

    #49852

    Purpose

    Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Expression

    Mammalian

    Mutation

    Contains several wobble mutations to confer resis…

    Promoter

    CMV

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pSUPER-retro-puro-Smad3

    Plasmid

    #89828

    Purpose

    Knockdown human Smad3 expression in human mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

    Insert

    SMAD3 shRNA (SMAD3 Human)
    

    Use

    Retroviral

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TTR F87E

    Plasmid

    #238075

    Purpose

    Bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    F87E

    Promoter

    T7

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCHC025 (∆CBBp)

    Plasmid

    #226203

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
    

    Use

    Synthetic Biology

    Mutation

    ∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpAp

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCHC036 (∆pHG1)

    Plasmid

    #226206

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for the pHG1 megaplasmid replication region
    

    Use

    Synthetic Biology

    Mutation

    ∆helD ∆repA ∆repB ∆parAB

    Available Since

    Nov. 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. EGFP-nCLC

    Plasmid

    #193564

    Purpose

    Expresses human Clathrin Light Chain B (neuronal isoform) tagged with mEGFP in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Neuronal isoform

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. nCLC-ShadowY

    Plasmid

    #193565

    Purpose

    Expresses human Clathrin Light Chain B (neuronal isoform) tagged with ShadowY in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Tags

    ShadowY

    Expression

    Mammalian

    Mutation

    Neuronal isoform

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSW031

    Plasmid

    #231144

    Purpose

    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

    Depositor

    Philip Cole

    Article

    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

    Insert

    S. aureus Sortase A mutant
    

    Tags

    Nostoc punctiform split intein C-terminal fragmen…

    Expression

    Bacterial

    Mutation

    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

    Promoter

    T7

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFPC2-CB2 SH3-

    Plasmid

    #68822

    Purpose

    EGFP tagged Collybistin expression in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

    Insert

    Collybistin (Arhgef9 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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