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pYZ_3WJrep_N20_switch

Plasmid

#132722

Purpose

T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Publication

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N20_switch

Expression

Bacterial

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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TP1376

Plasmid

#252368

Purpose

Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag

Depositor

Tino Pleiner

Publication

Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.

Insert

anti-EMC Nanobody G9

Tags

ALFA-GFP-SUMOEu and His10

Expression

Bacterial

Mutation

Contains engineered nanobody variant with ALFA ta…

Available since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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pVG144

Plasmid

#227760

Purpose

Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Val121Met mutant

Promoter

PGK1pr

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG124

Plasmid

#227758

Purpose

Expresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Thr83Ser mutant

Promoter

PGK1pr

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCK389

Plasmid

#206791

Purpose

Sp.dCas9, pTet.MCP-SoxS, J23119.gRNA

Depositor

James Carothers

Publication

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Inserts

dCas9
MCP-SoxS
scRNA

Promoter

J23119 and Tet

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDA303.DA9.1B.mRFP

Plasmid

#206802

Purpose

mRFP with synthetic promoter HP3 (from Figure S21)

Depositor

James Carothers

Publication

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mRFP

Promoter

DA9.1B (HP3)

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDIRECT_26K

Plasmid

#91152

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TALEN

Use

TALEN

Expression

Plant

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDIRECT_21J

Plasmid

#91132

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Expression

Plant

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pTC362

Plasmid

#91216

Purpose

protoplast vector for targeted deletion of 6 genes in tomato

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting 6 tomato genes

Use

CRISPR

Expression

Plant

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A3601

Plasmid

#91051

Purpose

Module A, Promoter: 35S, Gene: dAtCas9-VP64, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dAtCas9-VP64

Use

CRISPR

Promoter

35S

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDIRECT_37J

Plasmid

#91153

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDIRECT_23J

Plasmid

#91142

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDIRECT_22J

Plasmid

#91137

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAGD236

Plasmid

#219497

Purpose

pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

sfGFP, Eco1 msr, Eco1 RT

Expression

Bacterial

Promoter

T7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-lifeact-oROS-HT

Plasmid

#216420

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

Depositor

Andre Berndt

Publication

Lee et al bioRxiv 2024.02.06.579232

Insert

lifeact-oROS-HT

Expression

Mammalian

Promoter

CMV

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-oROS-HT

Plasmid

#216416

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.

Depositor

Andre Berndt

Publication

Lee et al bioRxiv 2024.02.06.579232

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pDisplay-oROS-HT

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJG486

Plasmid

#91195

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi1+donor

Use

CRISPR

Expression

Plant

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTaCas9-D10A_5

Plasmid

#91170

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-dead_1

Plasmid

#91165

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDIRECT_21D

Plasmid

#91131

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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