Addgene

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pAAV-Puro_siKD

Plasmid

#86695

Purpose

Tetracycline inducible expression of shRNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

H1 TO

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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LLP786_SpyCatcher-EZH2-FL-CO

Plasmid

#211779

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-KRAB

Use

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

Plasmid

#227246

Purpose

All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

Depositor

Jae Seong Lee

Article

Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

Inserts

Tet-On 3G transactivator protein
Enhanced green fluorescent protein

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1α and pTRE3G

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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NRXN-bio-His

Plasmid

#51810

Purpose

Expresses full-length Neurexin-1-alpha precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

NRXN (NRXN1 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG

Plasmid

#70183

Purpose

Inducible expression of guide RNA with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

H1-Tet-sgrna cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSB-TRE-CRISPRoff-EF1A-TetOn

Plasmid

#203355

Purpose

Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

Depositor

Jonathan Brunger

Article

Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

Insert

CRISPRoff-TetOn

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-q ONE-GO

Plasmid

#189738

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-q ONE-GO
GNAQ (Gnaq Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PKCdelta-EGFP

Plasmid

#178856

Purpose

Mammalian expression of PKCδ fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ (PRKCD Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-E

Plasmid

#78852

Purpose

Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

Depositor

Phillip Abbosh

Article

Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Cas9-P2A-Puro

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

Plasmid

#214875

Purpose

Empty lentiviral backbone for guide array cloning

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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ColorfulCell

Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Sst44-GFP

Plasmid

#172310

Purpose

SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

Depositor

Michael Greenberg

Article

Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

Insert

Sst44

Use

AAV

Tags

Expression

Mutation

Promoter

pB-Globin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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PLJR962

Plasmid

#115162

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMEH31 Lamp2b HA

Plasmid

#168208

Purpose

Mammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

Lamp2b (LAMP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i3 ONE-GO

Plasmid

#189737

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i3 ONE-GO
GNAI3 (Gnai3 Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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FUCas9Cherry

Plasmid

#70182

Purpose

Expresses Cas9 with fluorescent Cherry reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

humanised S.pyogenes cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AD101

Plasmid

#131402

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD101

Use

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGCaMP6m-N3-TPC2

Plasmid

#80147

Purpose

GCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real time

Depositor

Santiago Di Pietro

Article

Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

Insert

TPCN2 (TPCN2 Human)

Use

Tags

GCaMP6m

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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B2345_BTB2x-mCherry

Plasmid

#215097

Purpose

Expresses BCL6 BTB(1-129)-BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB-BTB (BCL6 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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SPARC-bio-His

Plasmid

#52078

Purpose

Expresses full-length Secreted protein acidic and rich in cysteine precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

SPARC (SPARC Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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