We narrowed to 2,568 results for: PGK
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pSR1057
Plasmid#208742PurposeCharacterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)DepositorInsertPPgk
UseSynthetic BiologyExpressionBacterialPromoterPPgkAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2362_Tier3(SB)-rtTA-PuroR
Plasmid#169658PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro
Plasmid#169665PurposeTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPhCMV / PPGK / RPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2361_Tier3(SB)-rtTA-YPet_PuroR
Plasmid#169657PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2363_Tier3(PB)-rtTA-YPet_PuroR
Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1
Plasmid#194703PurposeInducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)DepositorInsertRPS2 (RPS2 Human)
UseLentiviralTagsAPOBEC1-HA-P2A-mRubyExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_WT
Plasmid#183112PurposeExpresses flag-tagged p53 in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-LTR-dCas9-VP64-BFP
Plasmid#46912PurposeHuman expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFPDepositorInsertsdCas9-VP64-BFP fusion
Puromycin resistance
UseCRISPR and RetroviralTags3xNLS, BFP, and VP64 domainExpressionMammalianPromoterLTR and PGKAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFB2B
Plasmid#195284PurposeBicistronic HSV-TK and Puromycin resistance for positive/negative selectionDepositorInsertHSV-TK 2A Puro
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-IKKbeta (S177E,S181E)-pcw107
Plasmid#64609Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertIKBKB
UseLentiviralTagsFLAGMutationS177E, S181EPromoterPGKAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB2F
Plasmid#195289PurposeBicistronic HSV-TK and mScarlet resistance for positive/negative selectionDepositorInsertHSV-TK 2A mScarlet
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB2C
Plasmid#195285PurposeBicistronic HSV-TK and Hygromycin resistance for positive/negative selectionDepositorInsertHSV-TK 2A Hygro
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Rheb (Q64L)-pcw107
Plasmid#64607Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Mkk7-JNK2 fusion-pcw107
Plasmid#64618PurposeMkk7-JNK2 fusion - barcoded activating construct of JNK pathwayDepositorInsertMap2k7 linker MAPK9
UseLentiviralTagsFLAGMutationmouse-human fusionPromoterPGKAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_R248W
Plasmid#183114PurposeExpresses flag-tagged p53 with R248W mutation in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged arginine 248 to tryptophanPromoterEF1aAvailable SinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB2G
Plasmid#195290PurposeBicistronic HSV-TK and mTagBFP resistance for positive/negative selectionDepositorInsertHSV-TK 2A mTagBFP
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-LTR-dCas9-p65AD-BFP
Plasmid#46913PurposeHuman expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFPDepositorInsertsdCas9-p65AD-BFP fusion
Puromycin resistance
UseCRISPR and RetroviralTags2xNLS, BFP, and VP64 domainExpressionMammalianPromoterLTR and PGKAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_R248W+P278A
Plasmid#183115PurposeExpresses flag-tagged p53 with both R248W and P278A mutations in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged proline 278 to alanine and arginine 248 t…PromoterEF1aAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV p2GM AMPK alpha2hp1 alpha1hp1
Plasmid#89492PurposeshRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expressionDepositorInsert2 hairpins against mouse/human AMPKalpha1 and alpha2
UseRetroviralExpressionMammalianPromoterU6 (The GFP-Puro is from a PGK promoter; the shRN…Available SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only
