We narrowed to 12,966 results for: lic
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Plasmid#155156PurposeExpresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
MutationMlaF_K47APromoterT7 promoterAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2077
Plasmid#155157PurposeExpresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
MutationMlaF_E170QPromoterT7 promoterAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2078
Plasmid#155158PurposeExpresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
MutationMlaF_Y261AAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL2079
Plasmid#155159PurposeExpresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
MutationMlaF_L265AAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1244
Plasmid#155138PurposeExpresses MlaFED operon in E. coliDepositorInsertmlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon
PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Yes-10kb
Plasmid#112996PurposeP element vector for transposon insertion of the 10Kb Drosophila Amplicon in Follicle Cells (DAFC-62D) into Drosophila genomeDepositorInsert10Kb DAFC-62D amplicon
UseP insertion vectorAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSABAD518
Plasmid#46374DepositorInsertTvoVMA intein, inactive
TagsH6-GB1 and SH3ExpressionBacterialMutationN186A, T+1A mutations, inactivePromoterpBADAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG-deltaA
Plasmid#33151DepositorInsertRP51A-synthetic minimal intron (RPS17A Budding Yeast)
TagsLacZExpressionYeastMutationThe RP51A sequence was modified by digestion with…Available SinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a207-368.wt
Plasmid#13531DepositorInsertB. subtilis DEAD-box helicase YxiN second domain
Tagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 1-206 and 369-479Available SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-NHX1-HaloTagx2
Plasmid#177107PurposeExpresses Nhx1-HaloTagx2 in yeast cellsDepositorInsertNHX1 (NHX1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-NHX1-iGFP
Plasmid#177108PurposeExpresses Nhx1-iGFP in yeast cellsDepositorInsertNHX1 (NHX1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
Tags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
ERM-APEX2
Plasmid#79055PurposeSoybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.DepositorInsertAPEX2 anchored to the cytosolic face of the ER membrane
UseLentiviralTagsResidues 1-27 of P450 oxidase 2C1 and V5ExpressionBacterial and MammalianPromoterCMV immearly promotorAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB_mU6_enh-gRNA_Puro-T2A-BFP
Plasmid#235571PurposeEnhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.DepositorInsertsgRNA backbone
UseCRISPRAvailable SinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianPromoterCMVAvailable SinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTP1172
Plasmid#104161PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAL1
Plasmid#197285PurposeShuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195DepositorInsertOrigin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
UseSynthetic Biology; Bacterial and yeast cloningAvailable SinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Lifeact_Halo_T2A_EGFP
Plasmid#135445PurposeMamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFPDepositorInsertLifact-HaloTag7 and EGFP
TagsLifactExpressionMammalianPromoterCMV/TetOAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI
Plasmid#115268PurposeExpresses EiCsm6 from a SUMO-tagged bacterial expression constructDepositorInsertEiCsm6
TagsSUMOExpressionBacterialAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only