We narrowed to 4,232 results for: pCMV

1. pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

   Plasmid

   #178868

   Purpose

   Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3XFlag pCMV5-TOPO TAZ (∂393)

   Plasmid

   #24812

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Contains ONLY amino acids 1-393. (No PDZ binding…

   Available Since

   June 3, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV Flag dPDZ-PTEN (1-400)

   Plasmid

   #22232

   Depositor

   Hong Wu

   Article

   Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

   Insert

   PTEN (Pten Mouse)
   

   Tags

   Flag

   Expression

   Bacterial and Mammalian

   Mutation

   PDZ binding domain deleted. Contains amino acids …

   Available Since

   March 19, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-FLAG TIF1gamma M (456-887)

   Plasmid

   #15735

   Depositor

   Joan Massague

   Article

   He et al Cell. 2006 Jun 2. 125(5):929-41.

   Insert

   TIF1 gamma M (TRIM33 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Middle region (amino acids 456-887).

   Available Since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV Gab1(PRD)-mCherry-HOTag3

   Plasmid

   #190026

   Purpose

   Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

   Depositor

   Lukasz Bugaj

   Article

   Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

   Insert

   Gab1(PRD) CluMPS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-Flag-YAP-4SA(S109)

   Plasmid

   #33081

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

   Insert

   YAP (YAP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S61A, S127A, S164A, S381A

   Promoter

   CMV

   Available Since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

   Plasmid

   #174140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

   Promoter

   CMV and T7

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV5 Flag-Smad1 (4SP/AP+AAVA)

   Plasmid

   #14955

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

   Insert

   Smad1 (4SP/AP+AAVA) (SMAD1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Four Serine to Alanine mutations in the linker re…

   Available Since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-TNT-CFP-STIM1-C227W

    Plasmid

    #68403

    Purpose

    Expresses a STIM1 gain-of-function mutant in mammalian cells

    Depositor

    Yubin Zhou

    Article

    Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

    Insert

    CFP-STIM1 (STIM1 Human)
    

    Tags

    Cyan Fluorescent Protein (CFP)

    Expression

    Mammalian

    Mutation

    Changed Cysteine 227 to Tryptophan; CFP was inser…

    Promoter

    CMV

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-Flag-YAP-S163/164A

    Plasmid

    #33089

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S163A, S164A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-Tag2-Sox9 T236A/T240A

    Plasmid

    #111458

    Purpose

    Transient expression of Flag-tagged human Sox9 with T236A/T240A mutation

    Depositor

    Sharon Pine

    Article

    Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

    Insert

    Sox9 (SOX9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T236A and T240A

    Promoter

    CMV

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-RA-HRas-IRES2-Lyn-iRFP682

    Plasmid

    #180584

    Purpose

    RA-Hras and Lyn-iRFP682

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    RA-Hras and Lyn-iRFP682
    

    Expression

    Mammalian

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

    Plasmid

    #115137

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy, Keith Joung

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA2.1 anti-CRISPR protein
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-bArrestin2-TevC-P2A-TdTomato

    Plasmid

    #89869

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (please see depositor comments …

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV5-Flag-EPLIN delta N-term

    Plasmid

    #40932

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-220

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

17. p231 pCMVe-betaAc-STOP-luc

    Plasmid

    #12463

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CMVe-betaAc-STOP
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-deltaR8.2(A14C,E45C,A92E)

    Plasmid

    #79048

    Purpose

    mammalian expression, lentiviral packaging

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV HA hRB delta CDK + S780

    Plasmid

    #58915

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S780 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-NES-MBP-cATOM(Ras)

    Plasmid

    #209711

    Purpose

    Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NES-MBP-cATOM(Ras)
    

    Tags

    NES-MBP

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge