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We narrowed to 2,405 results for: dcas9

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Showing: 2121 - 2140 of 2405 results

  1. pEG302 5aa SunTag VP64 gRNA17 (FWA)

    Plasmid
    #115482
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    115482-plasmid-map-sequence-id-238819

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  3. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  4. pSR2001

    Plasmid
    #222626
    Purpose
    Sp-dCas9 destination vector, version 2
    Depositor
    Lauren Andrews
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available since
    June 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    222626-plasmid-map-sequence-id-441109

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  5. KTK_095

    Plasmid
    #180524
    Purpose
    dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly
    Depositor
    Tom Ellis
    Publication
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    180524-plasmid-map-sequence-id-359542

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  6. pETM6-1D4-mCherry

    Plasmid
    #73423
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1D4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1D4 (orthogonal T7-lac variant)
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73423-plasmid-map-sequence-id-135323

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  7. pETM6-4A6-mCherry

    Plasmid
    #73424
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4A6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4A6 (orthogonal T7-lac variant)
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73424-plasmid-map-sequence-id-135324

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  8. pETM6-1E4-mCherry

    Plasmid
    #73426
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1E4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1E4 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73426-plasmid-map-sequence-id-135326

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  9. pETM6-1B6-mCherry

    Plasmid
    #73420
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1B6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1B6 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73420-plasmid-map-sequence-id-135320

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  10. pETM6-3H5-mCherry

    Plasmid
    #73419
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3H5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3H5 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73419-plasmid-map-sequence-id-135319

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  13. pEG302 5aa SunTag VP64 g4+g17 (FWA)

    Plasmid
    #119672
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    119672-plasmid-map-sequence-id-242023

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  16. pEG302 22aa SunTag VP64 gRNA17 (FWA)

    Plasmid
    #120250
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    120250-plasmid-map-sequence-id-238538

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  17. pJOG253

    Plasmid
    #80585
    Purpose
    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA
    Depositor
    Johannes Stuttmann
    Publication
    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1
    Inserts
    pnos:PAT-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80585-plasmid-map-sequence-id-151872

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  18. pSR3036

    Plasmid
    #222629
    Purpose
    Sp-dCas9 CRISPRi plasmid targeting yejL (b2187) in E. coli MG1655
    Depositor
    Lauren Andrews
    Insert
    gRNA spacer sequence targeting yejL
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PTac
    Available since
    June 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    222629-plasmid-map-sequence-id-441106

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  19. pSR3039

    Plasmid
    #222630
    Purpose
    Sp-dCas9 CRISPRi plasmid targeting ompF (b0929) in E. coli MG1655
    Depositor
    Lauren Andrews
    Insert
    gRNA spacer sequence targeting ompF
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PTac
    Available since
    June 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    222630-plasmid-map-sequence-id-441107

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  20. pSR3030

    Plasmid
    #222627
    Purpose
    Sp-dCas9 CRISPRi plasmid targeting ompR (b3405) in E. coli MG1655
    Depositor
    Lauren Andrews
    Insert
    gRNA spacer sequence targeting ompR
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PTac
    Available since
    June 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    222627-plasmid-map-sequence-id-441108

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Showing: 2121 - 2140 of 2405 results