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pHHM.X513-iNicSnFR2-(CC90)

Plasmid

#124836

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Publication

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR2-(CC90)

Tags

HA tags and His Tag

Expression

Bacterial

Mutation

NA

Promoter

T7

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV TRE3G:jGCaMP8s

Plasmid

#239673

Purpose

jGCaMP8s expressed under a TRE3G (tet responsive element) promoter.

Depositor

Marianne Fyhn

Publication

Otero-Coronel et al bioRxiv 2026.05.28.728595

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

TRE3G

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV TRE3G:jGCaMP8f

Plasmid

#239674

Purpose

jGCaMP8f expressed under a TRE3G (tet responsive element) promoter.

Depositor

Marianne Fyhn

Publication

Otero-Coronel et al bioRxiv 2026.05.28.728595

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

TRE3G

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pYO1704

Plasmid

#252704

Purpose

Protein expression of GST-HsLC3B (1-120) in bacteria

Depositor

Roger Williams

Publication

van Vliet et al J Cell Biol. 2026 Mar 2;225(3):e20250708

Insert

GST-HsLC3B (1-120) (MAP1LC3B Human)

Expression

Bacterial

Mutation

1-120

Available since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV EfIa DIO CaBLAM

Plasmid

#244229

Purpose

Cre dependent bioluminescent reporter for calcium signaling

Depositor

Ute Hochgeschwender

Publication

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Inserts

mNeonGreen
CaBLAM

Use

AAV

Available since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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EF_tTpEF2

Plasmid

#139951

Purpose

Diatom terminator

Depositor

Raffaela Abbriano

Publication

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

Thalassiosira pseudonana EF2 terminator

Use

Synthetic Biology

Available since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AC_pTpEF2

Plasmid

#139938

Purpose

Diatom promoter

Depositor

Raffaela Abbriano

Publication

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

Thalassiosira pseudonana EF2 promoter

Use

Synthetic Biology

Available since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pNK845

Plasmid

#219746

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens

Depositor

Alexander Mishin

Publication

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal luciferase

Use

Luciferase and Synthetic Biology

Mutation

I3S, N4T, F11L, I63T, T99P, T192S, A199P

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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OM14-2EGFP-UG74-ter

Plasmid

#184758

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

OM14 (OM14 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2EGFP-ter-NAT

Plasmid

#184764

Purpose

Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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ACH1-2EGFP-ter-URA3

Plasmid

#184769

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ACH1 (ACH1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SYS1-2EGFP-ter-URA3

Plasmid

#184771

Purpose

Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SYS1 (SYS1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_ER

Plasmid

#182818

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_PM

Plasmid

#182819

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_cyto

Plasmid

#182820

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iFluoxSnFR_ER

Plasmid

#182821

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_ER

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iFluoxSnFR_PM

Plasmid

#182822

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_PM

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iFluoxSnFR_cyto

Plasmid

#182823

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_cyto

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P40

Plasmid

#188057

Purpose

Expression of BLZinCh-P40, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Publication

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P40

Expression

Mammalian

Promoter

CMV promotor

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P50

Plasmid

#188058

Purpose

Expression of BLZinCh-P50, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Publication

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P50

Expression

Mammalian

Promoter

CMV promotor

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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