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Showing: 1 - 20 of 226 results
  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Updated
    April 14, 2021, 3:18 p.m.
    ...practical due to the low intensity of luminescent proteins. To make luminescent imaging a reality, Addgene depositor...with Venus. This luminescent technology is based on the principle of bioluminescence resonance energy ... a few drawbacks associated with luminescent imaging: A luminescent substrate is required for imaging...Researchers have long been interested in using luminescence to get around these issues, but this solution...fusion proteins. Solving the problems of low luminescence By itself, Renilla luciferase has a low quantum... hasn’t stopped some scientists from using chemiluminescent proteins for imaging, but it has precluded...orange Nano-lanterns and showed that these three luminescent proteins can be used together in cells. As Nano-lanterns...
  2. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Updated
    Nov. 26, 2020, 3:42 p.m.
    ...eScience Labs as part of their curriculum by either taking existing curriculum and customizing it for schools...the entire curriculum development team at eScience Labs. She still gets to write curriculum, but has taken...Caitlin’s take on her job as an SME: “I write curriculum for students taking science courses online, which...student the best possible experience.” Managing curriculum development About a year after Caitlin was hired...
  3. Optogenetics Guide

    Type
    Guide
    ...sequestered Lumitoxin Peptide toxin for blocking K + (Kv) channels fused to LOV2 photoswitch. Illumination causes...expression of the opsin and the area that is being illuminated. There are several different ways to control ...2006. Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci...
  4. Molecular Biology Reference

    Type
    Guide
    ...acquire NGS but one of the most commonly used is the Illumina NGS platform. This is the platform used by Addgene...sequencing partner, seqWell . The actual process of Illumina NGS is not that different from Sanger sequencing...modified fluorescently tagged nucleotides. During illumina NGS, a long piece of DNA is first fragmented into...regular base and thus do not halt the reaction. Illumina NGS, therefore does not require any “normal” bases...
  5. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Emission: 649 mPlum-CAF1-C-10 Data Supplement mPlum-Calnexin-N-14 Localization: Endoplasmic Reticulum, Excitation...Emission: 649 mPlum-Cx43-7 Data Supplement mPlum-CytERM-N-17 Localization: Endoplasmic Reticulum, Excitation...Supplement mPlum-ER-3 Localization: Endoplasmic Reticulum, Excitation: 590, Emission: 649 mPlum-ER-3 Data...Supplement mPlum-ER-5 Localization: Endoplasmic Reticulum, Excitation: 590, Emission: 649 mPlum-ER-5 Data...Supplement mPlum-Peroxisomes-2 Localization: Peroxisomes, Excitation: 590, Emission: 649 mPlum-Peroxisomes...Supplement mPlum-pBAD Localization: Protein Expression Vector , Excitation: 590, Emission: 649 mPlum-pBAD Data... Supplement mPlum-N1 Localization: N1 Cloning Vector, Excitation: 590, Emission: 649 mPlum-N1 Data Supplement...
  6. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Endoplasmic Reticulum KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic Reticulum KDEL ...Endoplasmic Reticulum ER retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention...Endoplasmic Reticulum BiP ER signal sequence BFP Gia Voeltz 61807 Rtn4a-GFP Endoplasmic Reticulum Rtn4a AcGFP...Endoplasmic Reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic Reticulum Sec61 BFP Gia...Endoplasmic Reticulum CytERM mScarlet-I Dorus Gadella 85067 pCytERM_mScarlet-H_N1 Endoplasmic Reticulum CytERM...Endoplasmic Reticulum CytERM mScarlet Dorus Gadella 158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin...Proteins are first translated at the endoplasmic reticulum, then are transported to the Golgi apparatus in...
  7. Brain Initiative Plasmid Collection

    Type
    Collection
    ...calcium-modulated bioluminescent indicator Lin 124097 pcDNA3.1-Blue_CaMBI Blue calcium-modulated bioluminescent indicator...and enhanced yellow fluorescent protein for bioluminescent optogenetics Hochgeschwender 114100 pAAV-hSyn-iLMO...fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced...enhanced yellow fluorescent protein for bioluminescent optogenetics Hochgeschwender 114101 pAAV-hSyn-LMO4 (...and enhanced yellow fluorescent protein for bioluminescent optogenetics Hochgeschwender 114102 pAAV-hSyn-iLMO4...and enhanced yellow fluorescent protein for bioluminescent optogenetics Hochgeschwender 114103 pAAV-Hb9...and enhanced yellow fluorescent protein for bioluminescent optogenetics Hochgeschwender 114104 pAAV-CAG-LMO3...
  8. Luciferase Plasmids

    Type
    Collection
    ... of experimental systems. LumiLuc and LumiScarlet : LumiLuc and LumiScarlet are ATP-independent luciferases... T-box site Blumberg 36246 TBRE-TK-Luc-WT Two copies of the Brachyury T-box site Blumberg 37099 psiCHECK2... Vector for LUMIER assay TEAD4 (Homo sapiens) Hata 128348 pCIneoLuc-PP1A Vector for LUMIER assay PP1A ...Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent Imaging with Nano-lanterns Collection Highlights...Luciferase is the enzyme responsible for the bioluminescence found in a diverse number of organisms, ranging... cells must be lysed in order to monitor the luminescence, making time-course experiments impossible. ...time course experiments possible, but its short luminescence half-life makes it too dim for many experimental...
  9. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...the editing of CNS disease genes Cell Reports , Volume 20 , Issue 12 , 2980 - 2991 pENTR221-H1-sgGFP1-...the editing of CNS disease genes Cell Reports , Volume 20 , Issue 12 , 2980 - 2991 Px461-Cas9n-Trp53-sgRNA-alpha...Maintenance of Formylmethionyl-tRNAs Mol. Cell (2018) Volume 69, Issue 4, p610–621.e5 pSECB sgMTHFD2L_2 sgRNA...Maintenance of Formylmethionyl-tRNAs Mol. Cell (2018) Volume 69, Issue 4, p610–621.e5 pLenti CRISPR sgSHMT2_...Maintenance of Formylmethionyl-tRNAs Mol. Cell (2018) Volume 69, Issue 4, p610–621.e5 pLenti CRISPR sgSHMT1_...
  10. Plasmids for Optogenetics Research

    Type
    Collection
    ....1-aDTX-lumitoxin Lumitoxin Mammalian mCherry Boyden 51689 pcDNA3.1-CONK1-lumitoxin Lumitoxin Mammalian...Mammalian mCherry Boyden 51691 pcDNA3.1-DTXK-lumitoxin Lumitoxin Mammalian mCherry Boyden 53095 PA-DAD-wt LOV...
  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Expression mPlum 590 649 4 4.5 100 min Monomer pQC mPlum IX - Mammalian Expression mPlum-N1 - Mammalian...Mammalian Expression mPlum-C1 - Mammalian Expression mPlum-pBAD - Bacterial Expression mRaspberry 598 625 13...
  12. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ... Cas9 pDREAM PX390 Flavobacterium columnare Cas9 FcCas9 (Synthetic) CMV Zhang Feng Zhang lab...bacterial Cas9 orthologs (unpublished) Flavobacterium columnare Cas9 pDREAM PX391 Sphaerochaeta globus...globus str. Buddy Cas9 pDREAM PX392 Azospirillum B510 Cas9 AzoCas9 (Synthetic) CMV Zhang Feng...Zhang lab bacterial Cas9 orthologs (unpublished) Azospirillum B510 Cas9 pDREAM PX393 Gluconacetobacter...Roseburia intestinalis Cas9 pDREAM PX396 Parvibaculum lavamentivorans Cas9 PlCas9 (Synthetic) CMV ...Zhang lab bacterial Cas9 orthologs (unpublished) Parvibaculum lavamentivorans Cas9 pDREAM PX398...the editing of CNS disease genes Cell Reports , Volume 20 , Issue 12 , 2980 - 2991 Transfer plasmid for...
  13. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP AICSDP-57 mEGFP ...SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus...tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell . 28(21):2854...
  14. Plasmids for Stem Cell Research

    Type
    Collection
    ...Cells Free of Viral Reprogramming Factors Cell, Volume 136, Issue 5, 964-977, 6 March 2009 FUW-tetO-lox-hOCT4...Cells Free of Viral Reprogramming Factors Cell, Volume 136, Issue 5, 964-977, 6 March 2009 FUW-tetO-lox-hSOX2...Cells Free of Viral Reprogramming Factors Cell, Volume 136, Issue 5, 964-977, 6 March 2009 pCAGMKOSiE ...palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc Natl Acad Sci U S A. 2015 Apr ...
  15. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...2 O to final volume of 50 μl. This reaction may be scaled down to a smaller final volume if necessary....contains 50 μl cell culture media per well for a final volume of 150 μl. This facilitates screening by allowing...Add an additional 100 μl to each well for a total volume of 200 μl. Wait 24 - 72 hr to allow cells to grow...
  16. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... Cas9 pDREAM PX390 Flavobacterium columnare Cas9 FcCas9 (Synthetic) CMV Zhang Feng Zhang lab...bacterial Cas9 orthologs (unpublished) Flavobacterium columnare Cas9 pDREAM PX391 Sphaerochaeta globus...globus str. Buddy Cas9 pDREAM PX392 Azospirillum B510 Cas9 AzoCas9 (Synthetic) CMV Zhang Feng...Zhang lab bacterial Cas9 orthologs (unpublished) Azospirillum B510 Cas9 pDREAM PX393 Gluconacetobacter...Roseburia intestinalis Cas9 pDREAM PX396 Parvibaculum lavamentivorans Cas9 PlCas9 (Synthetic) CMV ...Zhang lab bacterial Cas9 orthologs (unpublished) Parvibaculum lavamentivorans Cas9 pDREAM PX398...the editing of CNS disease genes Cell Reports , Volume 20 , Issue 12 , 2980 - 2991 Transfer plasmid for...
  17. DNA Purification Without a Kit

    Type
    Blog Post
    Updated
    Feb. 12, 2020, 1:33 p.m.
    ...compared to fresh columns, regenerated columns had similar DNA yields. Additionally, columns regenerated up...times had similar DNA yields as fresh columns, suggesting that columns can be reused at least five times....Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit....bunch of unused buffers because you’ve run out of columns. Kit-less DNA purification methods avoid many ...without a kit as well as one way to reuse silica columns from DNA purification kits. Kit-free alkaline lysis...protocol from Addgene follows a similar workflow as a column-based plasmid extraction kit. First, you lyse the...available kits but instead of binding DNA to silica columns, it’s bound to glass syringe filters. Normally ...
  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Updated
    Nov. 24, 2019, 11:29 p.m.
    ... the lab has dubbed these fusions LumiFluor Reporters. The LumiFluor reporter constructs were designed...fluorescent donor for a bioluminescent donor, making BRET probes. Here the bioluminescent donor is unaffected...deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold brighter than our more common...wavelengths should be used. Researchers often use bioluminescence to overcome these issues as traditional luciferases...excites the fluor through a process known as bioluminescent resonance energy transfer (BRET). The fluor...a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two imaging...is an excellent BRET donor due to its bright luminescence. Intracellular Calcium Sensor Yang, J. et al...
  19. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Updated
    Jan. 5, 2021, 6:07 p.m.
    ...disadvantages of luminescence versus fluorescence.  Table 1: Properties of Luminescence versus Fluorescence...Secreted Luminous shrimp(Oplophorus gracilirostris) 455 19 Coelenterazine None Yes Luminous Japanese... the emission of photons. The most familiar bioluminescent organism for most people is the firefly (Photinus...surprisingly it is also the most commonly used bioluminescent reporter. This beetle emits a yellow-green ...as substrates. Production of photons by this bioluminescent reporter occurs slower than fluorescent-based...the different mechanisms to produce photons, chemiluminescent reporters are generally less bright than fluorescent...Fluorescence   Luminescence Fluorescence Source of Emitted Photons Chemical reaction High-energy photons...
Showing: 1 - 20 of 226 results