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Showing: 1 - 20 of 279 results

  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...practical due to the low intensity of luminescent proteins. To make luminescent imaging a reality, Addgene depositor...with Venus. This luminescent technology is based on the principle of bioluminescence resonance energy ... a few drawbacks associated with luminescent imaging: A luminescent substrate is required for imaging...Researchers have long been interested in using luminescence to get around these issues, but this solution...fusion proteins. Solving the problems of low luminescence By itself, Renilla luciferase has a low quantum... hasn’t stopped some scientists from using chemiluminescent proteins for imaging, but it has precluded...orange Nano-lanterns and showed that these three luminescent proteins can be used together in cells. As Nano-lanterns...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...scientists. Seeing inside these specimens has offered illumination on biochemical processes that are crucial in...85% of its original value after 60 minutes of illumination, highlighting its reliability for long-term ...to a specific stimuli, which leaves only a small volume of fluorophores in an excited state, effectively...

  4. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...eScience Labs as part of their curriculum by either taking existing curriculum and customizing it for schools...the entire curriculum development team at eScience Labs. She still gets to write curriculum, but has taken...Caitlin’s take on her job as an SME: “I write curriculum for students taking science courses online, which...student the best possible experience.” Managing curriculum development About a year after Caitlin was hired...

  5. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Endoplasmic Reticulum KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic Reticulum KDEL ...Endoplasmic Reticulum ER retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention...Endoplasmic Reticulum BiP ER signal sequence BFP Gia Voeltz 61807 Rtn4a-GFP Endoplasmic Reticulum Rtn4a AcGFP...Endoplasmic Reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic Reticulum Sec61 BFP Gia...Endoplasmic Reticulum CytERM mScarlet-I Dorus Gadella 85067 pCytERM_mScarlet-H_N1 Endoplasmic Reticulum CytERM...Endoplasmic Reticulum CytERM mScarlet Dorus Gadella 158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin...Proteins are first translated at the endoplasmic reticulum, then are transported to the Golgi apparatus in...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ... of experimental systems. LumiLuc and LumiScarlet : LumiLuc and LumiScarlet are ATP-independent luciferases...for assays of transcriptional regulation and bioluminescent reporters. Plasmid...Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent Imaging with Nano-lanterns Collection Highlights...Luciferase is the enzyme responsible for the bioluminescence found in a diverse number of organisms, ranging...delivered intracellularly in order to measure luminescence. Gaussia luciferase is secreted and more stable...time course experiments possible, but its short luminescence half-life makes it too dim for many experimental...luciferase engineered to use the red-shifted substrate Akalumine with significantly brighter signal and emission...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Expression mPlum 590 649 4 4.5 100 min Monomer pQC mPlum IX - Mammalian Expression mPlum-N1 - Mammalian...Mammalian Expression mPlum-C1 - Mammalian Expression mPlum-pBAD - Bacterial Expression mRaspberry 598 625 13...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein Sensor for Ex Vivo and In...sensors Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded... local calcium microdomain in the endoplasmic reticulum. Biophys Rep (2019) 5, 31–42 Pingyong Xu Magnesium...Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+ with genetically encoded sensors...7058. Marie-Eve Paquet , Robert Campbell Lactate Lindoblum green fluorescent biosensor for lactate Green ...for in vivo pH measurements in the endoplasmic reticulum. Sci Rep. 2018 Aug 10;8(1):11985. Eckhard Boles...

  9. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...2 O to final volume of 50 μl. This reaction may be scaled down to a smaller final volume if necessary....contains 50 μl cell culture media per well for a final volume of 150 μl. This facilitates screening by allowing...Add an additional 100 μl to each well for a total volume of 200 μl. Wait 24 - 72 hr to allow cells to grow...

  10. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP AICSDP-57 mEGFP ...SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus...

  11. Genetic Code Expansion

    Type
    Collection
    ...Badran 209746 pAMC070n1a12 ScTrpRS, Lum1PylRS, MmPylRS S. cerevisiae, Lum1, M. mazei Bacterial Ahmed Badran...jannaschii Bacterial Ahmed Badran 209741 pAB228r3 Mlum1RS Bacterial Ahmed Badran 209742 pAB228p PylRS M. ...

  12. Chemogenetics Plasmids

    Type
    Collection
    ...recently developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting...

  13. Serotype Testing AAV

    Type
    Collection
    ...for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 37825-AAV1.T 20 µL $ 140 Add to Cart...for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 50465-AAV1.T 20 µL $ 140 Add to Cart...

  14. Antibody Guide

    Type
    Collection
    ...performs a chemiluminescent reaction in the presence of several different substrates. The luminescence produced... protein extract by centrifugation or affinity columns. Mix samples with a primary antibody, which is ...

  15. Antibody Production

    Type
    Collection
    ...supernatant using Protein A or Protein G affinity columns. Formulation Buffer After affinity chromatography...antibodies undergo a buffer exchange using centrifugal columns. The final formulation buffer is phosphate-buffered...

  16. AAV Packaged on Request

    Type
    Collection
    ...serotype (AAV1, AAV2, AAV5, AAV8, or AAVrg) and volume (1 mL, 2.5 mL, or 5 mL). Currently, we can only...yields may vary by construct. Size Quantity Total Volume Minimum Titer Typical Titer Small 10 × 100 µL aliquots...

  17. Plant Plasmids and Resources

    Type
    Collection
    ...general and species-specific gene expression (see the column ‘promoter’ in the tables for species-specific tools...highlights plasmids that feature fluorescent or luminescent proteins for expression in plants. You can search...

  18. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Cortical subplate Striatum Pallidum Hypothalamus Cerebellum Whole brain Addgene ID Plasmid Allen Institute...WPRE3-BGHpA AiP1379 AiE2150m SYFP2 Purkinje cells Cerebellum 208141 pAAV-AiE0390m_3xC2-minBG-SYFP2-WPRE3-BGHpA...

  19. DNA Purification Without a Kit

    Type
    Blog Post
    ...compared to fresh columns, regenerated columns had similar DNA yields. Additionally, columns regenerated up...times had similar DNA yields as fresh columns, suggesting that columns can be reused at least five times....Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit....bunch of unused buffers because you’ve run out of columns. Kit-less DNA purification methods avoid many ...without a kit as well as one way to reuse silica columns from DNA purification kits. Kit-free alkaline lysis...protocol from Addgene follows a similar workflow as a column-based plasmid extraction kit. First, you lyse the...available kits but instead of binding DNA to silica columns, it’s bound to glass syringe filters. Normally ...

  20. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... the lab has dubbed these fusions LumiFluor Reporters. The LumiFluor reporter constructs were designed...fluorescent donor for a bioluminescent donor, making BRET probes. Here the bioluminescent donor is unaffected...deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold brighter than our more common...wavelengths should be used. Researchers often use bioluminescence to overcome these issues as traditional luciferases...excites the fluor through a process known as bioluminescent resonance energy transfer (BRET). The fluor...a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two imaging...is an excellent BRET donor due to its bright luminescence. Intracellular Calcium Sensor Yang, J. et al...
Showing: 1 - 20 of 279 results