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Showing: 1 - 20 of 163 results
  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...practical due to the low intensity of luminescent proteins. To make luminescent imaging a reality, Addgene depositor...with Venus. This luminescent technology is based on the principle of bioluminescence resonance energy ... a few drawbacks associated with luminescent imaging: A luminescent substrate is required for imaging...Researchers have long been interested in using luminescence to get around these issues, but this solution...fusion proteins. Solving the problems of low luminescence By itself, Renilla luciferase has a low quantum... hasn’t stopped some scientists from using chemiluminescent proteins for imaging, but it has precluded...orange Nano-lanterns and showed that these three luminescent proteins can be used together in cells. As Nano-lanterns...
  2. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...eScience Labs as part of their curriculum by either taking existing curriculum and customizing it for schools...the entire curriculum development team at eScience Labs. She still gets to write curriculum, but has taken...Caitlin’s take on her job as an SME: “I write curriculum for students taking science courses online, which...student the best possible experience.” Managing curriculum development About a year after Caitlin was hired...
  3. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...compared to fresh columns, regenerated columns had similar DNA yields. Additionally, columns regenerated up...times had similar DNA yields as fresh columns, suggesting that columns can be reused at least five times....Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit....bunch of unused buffers because you’ve run out of columns. Kit-less DNA purification methods avoid many ...without a kit as well as one way to reuse silica columns from DNA purification kits. Kit-free alkaline lysis...protocol from Addgene follows a similar workflow as a column-based plasmid extraction kit. First, you lyse the...available kits but instead of binding DNA to silica columns, it’s bound to glass syringe filters. Normally ...
  4. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... the lab has dubbed these fusions LumiFluor Reporters. The LumiFluor reporter constructs were designed...fluorescent donor for a bioluminescent donor, making BRET probes. Here the bioluminescent donor is unaffected...deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold brighter than our more common...wavelengths should be used. Researchers often use bioluminescence to overcome these issues as traditional luciferases...excites the fluor through a process known as bioluminescent resonance energy transfer (BRET). The fluor...a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two imaging...is an excellent BRET donor due to its bright luminescence. Intracellular Calcium Sensor Yang, J. et al...
  5. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...disadvantages of luminescence versus fluorescence.  Table 1: Properties of Luminescence versus Fluorescence...Secreted Luminous shrimp(Oplophorus gracilirostris) 455 19 Coelenterazine None Yes Luminous Japanese... the emission of photons. The most familiar bioluminescent organism for most people is the firefly (Photinus...surprisingly it is also the most commonly used bioluminescent reporter. This beetle emits a yellow-green ...as substrates. Production of photons by this bioluminescent reporter occurs slower than fluorescent-based...the different mechanisms to produce photons, chemiluminescent reporters are generally less bright than fluorescent...Fluorescence   Luminescence Fluorescence Source of Emitted Photons Chemical reaction High-energy photons...
  6. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    .... coli strains. Increase the volume Starting with a larger culture volume can improve plasmid yield. Our...yields. Why would volume make a difference? In the simplest of terms, more volume = more cells = more plasmid...plasmid. When increasing the culture volume, be aware that a higher volume of lysate passed over the binding... prep. Check the bacterial strain Increase the volume Improve the culture media Let it incubate Check... Our lab often doubles the volume of culture used for inoculation if we get inadequate plasmid DNA yields...purify! Additionally, if you've reached your lysate volume limits, the copy number of some origins of replication...indicate the number of cells present in the given volume.  Let oxygen in! Make sure your culture is getting...
  7. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...concentrate in a smaller volume. Consequently, you will end up with a considerably lower volume than with the unconcentrated...polyethyleneimine, calcium phosphate or liposomal methods), the volume of the reaction, and the timing and number of harvests...ultracentrifugation, centrifugation on a sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation. All...this in mind, if the experiment requires a certain volume of virus, consider scaling up by either increasing...require some initial optimization; cell number, media volume, and plasmid concentrations will need to be optimized...number, plasmid concentration, and experimental volumes accordingly. While you may need to make some final... you don’t anticipate that you will need large volumes of lentivirus routinely, you may opt for simply...
  8. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...restricting fluorescence to specific excitation volumes to enhance spatial contrast (i.e. optical sectioning... light exits the microscope objective evenly illuminating the entire (wide) field of view. Fluorescent...and focused onto a camera for visualization. Illuminating a sample in the opposite direction to fluorescence...fluorescence collection is referred to as “Epi” illumination. Therefore these are sometimes call Epi-fluorescence...location in the sample. As more than one hole is illuminated at a time, the image can be acquired more quickly...project excitation light through the entire axial volume of a sample. Therefore, when a large number of ...constantly exposed to light, not just those in the focal volume. This leads to more rapid photobleaching and a ...
  9. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...organized format.  Columns. You can choose from either a two, three, or four column style, and in all ...; the reader will navigate the poster from column to column left-to-right, which is the natural way that...success of this style is clear borders around your columns, and good spacing between them so that they are... some Powerpoint based templates with different column numbers for a good starting point. Large central...central box is then flanked by two equally-sized columns, where the introduction, methods, conclusions, ...horizontally. Instead of displaying your information as columns, you may choose to display it as rows. It can be...
  10. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...molecules within a volume. In many biological research situations, the relevant volume is that of the cell...molecules are homogenously distributed throughout the volume and can freely tumble in 720°. However, if the ...For a membrane POI localized to the endoplasmic reticulum, the normally spiderweb-like pattern of tubules...membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing FP is fused to the POI...describing the FPs above including molecular sizing columns and native gels fail to detect EGFP dimerization...control of secretory proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik is also..., M.V., et al., Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol, 1999. 17...
  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...Spelman focused a laser beam into a thin sheet to illuminate a fluorescent sample and captured the reflected... lens oriented perpendicular to the plane of illumination (i.e. light sheet).  This is in contrast to ...described the single-plane or selective-plane illumination microscope (SPIM). In order to improve axial...the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample surrounding....  One limitation of LSFMs using single side illumination, especially with large tissue, is that any obstacles...concentration of fluorophores) cast shadows along the illumination path. The shadows appear as stripes in the acquired... (5).  A way to get around this problem is to illuminate and acquire images of a sample from two opposing...
  12. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...incorporate more science and technology into the school curriculum. Our Parent Teacher Association (PTA) has been...been instrumental in supplementing that curriculum. It’s through the PTA that I recently started coordinating...Lab Middle school: supplementing the science curriculum Sharing my expertise with our district middle...tried contacting our school district’s science curriculum coordinator with minimal luck, but eventually...speaking on topics that would compliment the STEM curriculum. As it happened, I’d met some of the 8th grade...said “it was such a valuable extension to our curriculum and offered engaging examples of how understanding... who work hard maintaining a current, dynamic curriculum, would be hard-pressed to maintain the pace of...
  13. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...Luminescent molecules are very useful tools because we can easily detect and measure the light they emit...emit. Proteins that give off light include chemiluminescent proteins, like luciferases, as well as fluorescent...Protein (GFP). These molecules occur naturally in bioluminescent organisms, but their real power lies in the...interesting. Although humans have known about bioluminescent organisms and their characteristic radiance..., in the 1960s, Osamu Shimomura studied the bioluminescent properties observed in the crystal jellyfish...Lukyanov realized that, although they are not bioluminescent, some types of corals and anemones are vibrantly...
  14. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...to as many lab alumni as you can or schedule video/calls if they are not local. Alumni meetings can be... too long or do they leave prematurely? Do lab alumni stay in the same field taking projects with them...remaining close colleagues of the PI? Or do lab alumni leave the field or get pushed out by the PI? What...One scientist who followed my advice to interview alumni of the lab was told that the PI removed a woman... happen to you. As you speak to lab members and alumni, don’t ever disregard warning signs of unethical...supporting career development and growth. Have lab alumni gone into both academic and non-academic careers...
  15. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...kits and lesson plans adapted from her own MIT curriculum. Drawing on current questions being asked in ...field of STEM education worldwide. The BioBuilder curriculum is now taught in almost every state in the U....BioBuilder experience With its intentionally malleable curriculum, BioBuilder takes cutting-edge research projects... as a final project. BioBuilder’s open-access curriculum begins with students reading comic strips and...their hands-on experiments.  As a project-based curriculum, BioBuilder expands learning outcomes by emphasizing... by O’Reilly Media. BioBuilder's open-access curriculum available on Amazon Inspire.    Many thanks...
  16. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...YFP and use an affinity column for purification. After binding YFP to the column, you wash several times... use OptoNBs. You fill a column with OptoNB coated beads and wrap the column with blue LED lights. When...their target protein is altered by blue light illumination. This ability to reversibly bind and release...pathway. What’s next for OptoNBs? While OptoNBs illuminate a path to light-controlled protein binding, they...
  17. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...bind the IgG heavy chain. Running the serum over a column of resin crosslinked to protein A /G will bind ... antigen. In this case, the serum is run over a column containing the target protein, and any antibody...recognizing the target protein remains bound to the column while the rest of the proteins are eluted. Then...Then, the antibodies are dissociated from the column and from the target protein. Pre-adsorption Finally... pre-adsorb the antibodies, pass them through a column containing immobilized serum protein from other...-react with the serum proteins pass through the column, resulting in a polyclonal antibody population ...
  18. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...can be measured using a technique known as Bioluminescence Resonance Energy Transfer (BRET). BRET is similar...BRET is that the energy transfer is between a luminescent donor and a fluorescent acceptor. TRUPATH uses...measures heterotrimeric G protein dissociation by bioluminescence resonance energy transfer 2, or BRET2. With... constructs. Cells are then treated with the luminescent enzyme substrate coelenterazine, followed by ...endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2 will be...
  19. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...compensate for this, researchers can plate larger volumes of the outgrowth containing the transformants onto...the lacI/lacZ cassette, and illustrates how the volume of the 1 ml outgrowth spread on each transformation...fragments assembled Golden Gate Assembly protocol* (Volume of 1mL Outgrowth plated) Correct assemblies per...Efficiency of assemblies per plate using outgrowth volumes described in Figure 3, with calculated yields from...ºC incubations or cycling.  *Assembly reaction volumes were 20 μl (1, 12 fragments) or 25 μl (24 fragments...
Showing: 1 - 20 of 163 results