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MXS_PGK::CreERT2-bGHpA

Plasmid

#62444

Purpose

MXS_chaining vector with PGK::CreERT2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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neurod1:CaMPARI

Plasmid

#173479

Purpose

CaMPARI is driven by -5kb neurod1 promoter.

Depositor

John Rawls

Article

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

CaMPARI

Use

Zebrafish expression

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pEBFP2-Nuc

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pET11a-4SS-HEWL

Plasmid

#39233

Depositor

Harald Schwalbe

Article

Schlorb et al J Biomol NMR. 2005 Oct;33(2):95-104.

Insert

Hen egg white lysozyme (LYZ Chicken)

Expression

Bacterial

Mutation

N-terminal Met

Promoter

T7

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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attB-GT-mNeonGreen-linker::pUC-GW-Kan_mod

Plasmid

#244895

Purpose

Minicircle producer plasmid

Depositor

Andrew Bassett

Article

Migliori et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

mNeonGreen

Use

Unspecified

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mCherry

Plasmid

#195989

Purpose

Transgenesis backbone

Depositor

Christian Mosimann, Nicolas Rohner

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET29_LuxSit_i

Plasmid

#194299

Purpose

Bacterial expression of the de novo luciferase, LuxSit-i

Depositor

David Baker, Andy Yeh

Article

Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

Insert

LuxSit-i

Expression

Bacterial

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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scAAV-hSyn-Cre

Plasmid

#138253

Purpose

A self-complementary AAV vector expresses Cre recombinase from hSyn promoter.

Depositor

Li Zhang

Article

Zingg et al J Neurosci. 2020 Apr 15;40(16):3250-3267

Insert

Cre recombinase

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-rictor

Plasmid

#73387

Purpose

Expresses HA and YFP tagged Rictor

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCK054 pDEST exorh:mCerulean

Plasmid

#195985

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-destination

Plasmid

#60436

Purpose

Destination vector with PGK - Hygromycin selection marker

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hChR2(H134R)-EYFP

Plasmid

#20940

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CoiA1_CoiB

Plasmid

#208761

Purpose

Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiA1
CoiB

Tags

Hisx6 Tag and SUMO

Expression

Bacterial

Mutation

Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b PKA Cat

Plasmid

#14921

Depositor

Susan Taylor

Article

Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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H3K9me3Probe_FullLength

Plasmid

#186463

Purpose

Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

Depositor

Chongli Yuan

Article

Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

Insert

dimeric CDY1 (CDY1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-Klentaq

Plasmid

#157862

Purpose

Plasmid for the aTc-inducible expression of the Klentaq

Depositor

Andrew Ellington

Article

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

Klentaq

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAF020 p3E-2A-mCerulean

Plasmid

#195974

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCerulean

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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763 pME mApple

Plasmid

#195960

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pR-mAmetrine

Plasmid

#228598

Purpose

Mammalian expression of mAmetrine, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mAmetrine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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(+)-Limonene Synthase

Plasmid

#112230

Purpose

(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.

Depositor

Daniel Oprian

Article

Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17

Insert

(+)-Limonene Synthase

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

deleted amino acids 1-52

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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