We narrowed to 4,182 results for: biorxiv

1. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

   Plasmid

   #222989

   Purpose

   Bacterial expression of iGeoCas9(G) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   CL7_2xNLS_iGeoCas9(G)_2xNLS_His
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mc2 mCherry EF1a

   Plasmid

   #206225

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GALT-APEX2

   Plasmid

   #215554

   Purpose

   Stable, inducible expression in Golgi

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   APEX2-NES-GalT-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LBR-FKBP-GFP

   Plasmid

   #222413

   Purpose

   To express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al bioRxiv 2024.05.31.596797

   Insert

   LBR (LBR Human)
   

   Use

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

   Plasmid

   #202025

   Purpose

   Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GvpB S9G-R31L-R85L

   Promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

   Plasmid

   #216385

   Purpose

   Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Norrin (NDP Human)
   

   Use

   Baculovirus

   Tags

   GP64 signal sequence-MBP-3C and Rho1D4

   Expression

   Insect

   Mutation

   C93A/C95A/C131A

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH-nuc-roGFP2-GPXC71S

   Plasmid

   #191760

   Purpose

   Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   nuc-roGFP2-GPXC71S
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   GPX has no peroxidatic Cysteine 71, which is repl…

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-ConVERGD-ArchT(eGFP)-WPRE

   Plasmid

   #218760

   Purpose

   Provides AND intersectional (Cre+Flp) expression of ArchT-eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   ArchT
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-dSa-VPR mini.-syn pA

   Plasmid

   #99689

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mc2 neo EF1a

    Plasmid

    #206220

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-MBP-TEVpCS-ADGRF4 CTF-FLAG

    Plasmid

    #217697

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF4 (ADGRF4 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-384

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-MBP-TEVpCS-ADGRE1 CTF-FLAG

    Plasmid

    #217689

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE1 (EMR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-584

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mc2 blast EF1a

    Plasmid

    #206223

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFR-mTq2-antiFRET-P2A-jRCaMP1b

    Plasmid

    #191468

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jRCaMP1b
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mc2 mCherry

    Plasmid

    #206219

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TL021_lenti_CMV:SEP-GluA1

    Plasmid

    #192515

    Purpose

    Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

    Depositor

    Adam Cohen

    Article

    Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

    Insert

    SEP-GluA1
    

    Use

    Lentiviral

    Tags

    super ecliptic phluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mc2 neo

    Plasmid

    #206214

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pICH-chl-roGFP2-GPXC71S

    Plasmid

    #191703

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-redox(ro)GFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Peroxidatic Cysteine 71 replaced with Serine

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP ratAPOBEC1 L173A/G227A

    Plasmid

    #167169

    Purpose

    Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 173 to Alanine; changed Glycine 2…

    Promoter

    CMV promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ER-G-Ca-FLITS-KDEL

    Plasmid

    #191464

    Purpose

    Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    ER and KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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