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DNA-PKcs PQR>ala

Plasmid

#83319

Purpose

human DNA-PKcs PQR>ala

Depositor

Katheryn Meek

Publication

Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

5 phosphorylation sites in the PQR cluster substi…

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX1

Plasmid

#46848

Purpose

Vector modified from pBluescript SK(+) by adding more cloning sites

Depositor

Monica Hollstein

Publication

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Type

Empty backbone

Use

Cloning vector

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMKO.1-Puro-shPP2A-Aalpha

Plasmid

#15249

Depositor

William Hahn

Publication

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

PP2A regulatory subunit A, alpha isoform (PPP2R1A Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Publication

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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hFLT1

Plasmid

#83435

Purpose

human VEGFR1 with HA and FLAG tag

Depositor

Christie Thomas

Publication

Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

Insert

human VEGFR1 (FLT1 Human)

Tags

Flag and Myc

Expression

Mammalian

Mutation

HA-tag was inserted 30 AA downstream of the FLT1 …

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-3myc-6His-EZH2 21A

Plasmid

#42663

Depositor

Mien-Chie Hung

Publication

Cha et al Science. 2005 Oct 14;310(5746):306-10.

Insert

EZH2 (EZH2 Human)

Tags

3Myc and 6His

Expression

Mammalian

Mutation

S21A

Available since

July 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pUC8 human IFN-gamma

Plasmid

#17600

Depositor

Howard Young

Publication

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

interferon-gamma cDNA (IFNG Human)

Expression

Bacterial

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC7A5

Plasmid

#132197

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC7A5 (SLC7A5 Human)

Expression

Mammalian

Available since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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hRIP3 GFP D160N

Plasmid

#41386

Depositor

Francis Chan

Publication

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D160N)

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDNA3-Flag-Tpr

Plasmid

#60882

Purpose

Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells

Depositor

Vinay Nandicoori

Publication

Vomastek et al Mol Cell Biol. 2008 Nov;28(22):6954-66.

Insert

Tpr (TPR Human)

Expression

Mammalian

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GST-Hsp90 M(272-617)

Plasmid

#22482

Purpose

Please note this plasmid contains GST-Hsp90 residues 273-617.

Depositor

William Sessa

Publication

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90alpha M domain (HSP90AA2P Human)

Tags

GST

Expression

Bacterial

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pX330a dCas9-KRAB

Plasmid

#92361

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-KRAB

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-LacR-TopBP1

Plasmid

#31317

Depositor

Aziz Sancar

Publication

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Tags

FLAG and LacR

Expression

Mammalian

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pclbw-mitoTagRFP

Plasmid

#58425

Purpose

Mammalian expression of tagRFP targeted to the mitochondrial matrix

Depositor

David Chan

Publication

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mito-tagRFP

Use

Retroviral

Expression

Mammalian

Promoter

cmv-bactin

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pET28-Dnmt3a3L-sc27

Plasmid

#71827

Purpose

For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

Depositor

Albert Jeltsch

Publication

Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

Insert

Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)

Tags

6xHis tag

Expression

Bacterial

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCDNA3-V5-PTPN14-wild type

Plasmid

#61003

Purpose

Expresses wild type human PTPN14 in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Publication

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHMGCS-Luc (aka: pES8)

Plasmid

#14947

Depositor

Axel Nohturfft

Publication

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

HMG CoA synthase promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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pGL410_Ins2500

Plasmid

#49058

Purpose

Expresses luciferase under control of mouse insulin 2 gene promoter

Depositor

Kevin Ferreri

Publication

Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

Insert

mouse Ins2 promoter (Ins2 Mouse)

Expression

Mammalian

Promoter

Ins2 promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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TGFB1-bio-His

Plasmid

#52185

Purpose

Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TGFB1 (TGFB1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV5 MEKK2 WT

Plasmid

#12182

Depositor

Gary Johnson

Publication

Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

Insert

MEKK2 (Map3k2 Mouse)

Tags

HA

Expression

Mammalian

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article