We narrowed to 4,636 results for: pcr
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Plasmid#219709PurposeLevel 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pSPCH:PhiC31:tUBQ1-GG4
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T10 Circuit 1 GG4-Bxb1B-PhiC31B-GG6
Plasmid#219693PurposeLevel 0 to construct history-dependent target T2PBDepositorInsertGG4-Bxb1B-PhiC31B-GG6
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-I11 PhiC31-NLS GG1-GG4
Plasmid#195894PurposeLevel 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I12 PhiC31-NLS GG2-GG4
Plasmid#195895PurposeLevel 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj4
Plasmid#173131PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj4 (kcnj4 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
HoxC-downstream-5xTetO Donor
Plasmid#131341PurposeDonor template to insert 5xTet operator sequence into downstream of HoxC cluster.DepositorInsertHoxC-downstream-5xTetO Donor
UseCRISPR and Mouse TargetingAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6
Plasmid#219712PurposeLevel 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.DepositorInsertGG4-pGATA23-Bxb1-tUBQ10-GG6
UseSynthetic BiologyAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T7 Circuit 2 GG1-PhiC31P-bbs1-BxB1P-GG2
Plasmid#219692PurposeLevel 0 to construct history-dependent target T2BPDepositorInsertGG1-PhiC31P-bbs1-BxB1P-GG2
UseSynthetic BiologyAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T13 GG3-tRBCS-mCherry-NLS-GG4
Plasmid#219696PurposeLevel 0 to construct history-dependent targetDepositorInsertGG3-tRBCS-mCherry-NLS-GG4
UseSynthetic BiologyAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1
Plasmid#219697PurposeLevel 0 to construct history-dependent targetDepositorInsertGG0-tUBQ1-NLS-NeonGreen-GG1
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T6 Circuit 1 GG1-Bxb1P-bbs1-PhiC31P-GG2
Plasmid#219691PurposeLevel 0 to construct history-dependent target T2PBDepositorInsertGG1-Bxb1P-bbs1-PhiC31P-GG2
UseSynthetic BiologyAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-CD3D-274+5G>A
Plasmid#198023PurposeLV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in EcuadorDepositorInsertCD3D c.274+5G>A
UseLentiviralExpressionMammalianMutationCodon optimized (20 bp) on either end of cDNA for…Available SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-CD3D-275-2A>G
Plasmid#198024PurposeLV Expression of CD3D cDNA with mutation at c.275-2A>G used as a disease editing target for CD3d SCID mutation identified in JapanDepositorInsertCD3D c.275-2A>G
UseLentiviralExpressionMammalianMutationCodon optimized (20 bp) on either end of cDNA for…Available SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj20
Plasmid#173150PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj20 (si:ch211-113j13.2 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj13
Plasmid#173143PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj13 (kcnj13 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj21
Plasmid#173151PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj21 (zgc:162160 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 endAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj10a
Plasmid#173137PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj10a (kcnj10a Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnt2b
Plasmid#164964PurposeZebrafish kcnt2a gene CDSDepositorInsertkcnt2b (LOC565691 Zebrafish)
UsePcr cloning vectorAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK621
Plasmid#72388PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)DepositorInsertphosphoribosylamine-glycine ligase
TagsHis6ExpressionBacterialMutationcontains 3 silent mutations in codons 9-11 to fac…PromoterT7Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
L0I84 - GG0-pAHP6-GG1
Plasmid#219706PurposeLevel 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pAHP6-GG1
UseSynthetic BiologyMutationWT promoter with added golden gate linkersAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only