We narrowed to 14,580 results for: pET

1. pET28a-OuIAD(G853A)

   Plasmid

   #226969

   Purpose

   Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

   Depositor

   Emily Balskus

   Article

   Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

   Insert

   OuIAD(G853A)
   

   Use

   Tags

   His6 (on backbone)

   Expression

   Bacterial

   Mutation

   G853A

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a(+)-NEDD4L_W4-HECT

   Plasmid

   #187710

   Purpose

   Express his-tagged NEDD4L W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 543-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-542

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a(+)-NEDD4L_W3-W4-HECT_C874only

   Plasmid

   #187711

   Purpose

   Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-975 with mutations (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-491; mutations C702A, C776…

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28:LanIV-Streptomyces

   Plasmid

   #226250

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIV-Streptomyces)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-a(+)-6xHis-mScarlet-I(+5)

   Plasmid

   #219418

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Insert

   mScarlet-I(+5)
   

   Use

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   D125K, D186K, E216K, E238K

   Promoter

   T7

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET29b(+)_gp1(A11V)

   Plasmid

   #225131

   Purpose

   Phage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)

   Depositor

   Matej Butala

   Insert

   gp1 (A11V)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Alanine 11 to Valine

   Promoter

   T7 promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET20b-mPTER-H230F

   Plasmid

   #222195

   Purpose

   Bacterial expression of mouse PTER-H230F protein

   Depositor

   Jonathan Long

   Article

   Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

   Insert

   PTER (Pter Mouse)
   

   Use

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   H230F

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET20b-mPTER-H26F

   Plasmid

   #222192

   Purpose

   Bacterial expression of mouse PTER-H26F protein

   Depositor

   Jonathan Long

   Article

   Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

   Insert

   PTER (Pter Mouse)
   

   Use

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   H26F

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-dCas9-FCPF

   Plasmid

   #220133

   Purpose

   Expresses dCas9-FCPF in E.coli

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   dCas9-FCPF (cas9 S. pyogenes, Synthetic)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   FCPF sequence inserted on C-terminal

   Promoter

   T7

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28:LanIV-Chondromyces

    Plasmid

    #225078

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIV-Chondromyces)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28:LanI-101

    Plasmid

    #225065

    Purpose

    Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanI-101)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-a(+)-6xHis-mBlueberry(-9)

    Plasmid

    #219416

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Insert

    mBlueberry2(-9)
    

    Use

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    K137E, K199E, R234E

    Promoter

    T7

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET41-FeoB-OL2del

    Plasmid

    #216742

    Purpose

    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.

    Depositor

    Gordon Laurie

    Insert

    feoB (PA4358 P. aeruginosa (Bacteria))
    

    Use

    Tags

    8x His

    Expression

    Bacterial

    Mutation

    Outer loop 2 (OL2) with the sequence of [LEDSGYMA…

    Promoter

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET26b(modified)-FKBP12-Sm

    Plasmid

    #217004

    Purpose

    Expression of FKBP12-Linker-Small BiT

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    FKBP12-Linker-Small BiT
    

    Use

    Tags

    Split Nanoluciferase SmallBiT

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET26b(modified)-FRB-Lg

    Plasmid

    #216981

    Purpose

    Expression of FRB-Linker-Large BiT

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    FRB-Linker-Large BiT
    

    Use

    Tags

    Split Nanoluciferase LargeBiT

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-NIR-FbLAG30

    Plasmid

    #220748

    Purpose

    NIR-FbLAG30 expression in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-FbLAG30
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Bacterial

    Mutation

    For bacterial cytoplasmic expression, the pelB si…

    Promoter

    T7

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-SpyCatcher -AVI

    Plasmid

    #223635

    Purpose

    Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    AVI tag and His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SUMO-LPQTG-pET

    Plasmid

    #221851

    Purpose

    Expresses SUMO-2 with sortase motif and His tag

    Depositor

    Artur Krężel

    Article

    Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

    Insert

    SUMO-2 (SUMO2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gln88Leu, Gln89Pro

    Promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET20b-mPTER-E169A

    Plasmid

    #222190

    Purpose

    Bacterial expression of mouse PTER-E169A protein

    Depositor

    Jonathan Long

    Article

    Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

    Insert

    PTER (Pter Mouse)
    

    Use

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    E169A

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET20b-mPTER-D298A

    Plasmid

    #222191

    Purpose

    Bacterial expression of mouse PTER-D298A protein

    Depositor

    Jonathan Long

    Article

    Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

    Insert

    PTER (Pter Mouse)
    

    Use

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    D298A

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge