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gabra5_R (TAL3015)

Plasmid

#36007

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gabra5 (gabra5 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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gabra5_L (TAL3014)

Plasmid

#36006

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting gabra5 (gabra5 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrR1090A-HisB

Plasmid

#32507

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR )

Tags

HISB

Expression

Mammalian

Mutation

R 1090 to A

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
Ube2c

Plasmid

#31418

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2C Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
WDR5 (2H9M)

Plasmid

#25228

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

WDR5_01 (WDR5 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pDONR223-DGKG

Plasmid

#23529

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DGKG (DGKG Human)

Use

Gateway Cloning

Mutation

C425G, A477G

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag DGKG

Plasmid

#20475

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

DGKG (DGKG Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pBS cGli2 (CT#673)

Plasmid

#13955

Depositor

Cliff Tabin

Article

Schweitzer et al Mech Dev. 2000 Nov . 98(1-2):171-4.

Insert

Gli2 (GLI2 Chicken)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBS cGli3 (CT#34)

Plasmid

#13953

Depositor

Cliff Tabin

Article

Schweitzer et al Mech Dev. 2000 Nov . 98(1-2):171-4.

Insert

Gli3 (GLI3 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pBabe hygro human Frataxin

Plasmid

#11504

Depositor

Ronald Kahn

Article

Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Frataxin (FXN Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pPS2169

Plasmid

#1364

Depositor

Pamela Silver

Article

Stage-Zimmermann et al Mol Biol Cell 2000 Nov;11(11):3777-89.

Insert

RPL11B (RPL11B Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pPS2167

Plasmid

#1365

Depositor

Pamela Silver

Article

Stage-Zimmermann et al Mol Biol Cell 2000 Nov;11(11):3777-89.

Insert

RPL11B (RPL11B Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
RB-409-815-pGex2T

Plasmid

#21784

Depositor

William Kaelin

Article

Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 409-815 of wt Rb …

Available Since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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PIK3CB gRNA (BRDN0001147768)

Plasmid

#76194

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CB (PIK3CB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALPK1 gRNA (BRDN0001146195)

Plasmid

#76082

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALPK1 (ALPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Actin-7

Plasmid

#55085

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
p199 Dync1i2.C (Ex1a)

Plasmid

#26449

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
Str-KDEL_SBP-EGFP-LAMP2

Plasmid

#222321

Purpose

Synchronize the trafficking of LAMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A-PIK3CA E545K

Plasmid

#180032

Purpose

Entry vector of PIK3CA E545K for gateway cloning.

Depositor

Xin Chen

Article

Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

Insert

PIK3CA E545K (PIK3CA Human)

Use

Gateway Cloning

Tags

HA tag

Expression

Mammalian

Mutation

E545K

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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ABE8r-R153del

Plasmid

#208296

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-R153del-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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