We narrowed to 4,182 results for: biorxiv

1. pM-mOrange2

   Plasmid

   #228627

   Purpose

   Mammalian expression of mOrange2 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

   Insert

   mOrange2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pM-mRuby3

   Plasmid

   #228628

   Purpose

   Mammalian expression of mRuby3 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R

   Plasmid

   #228722

   Purpose

   Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEJS2702-CMV-iNme2-ABE8e-nt

   Plasmid

   #228723

   Purpose

   Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with iNme2 nCas9 (D16A)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

   Plasmid

   #228721

   Purpose

   Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEJS2703-CMV-iNme2Smu-ABE8e-nt

   Plasmid

   #228724

   Purpose

   Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with iNme2Smu nCas9 (D16A)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R

   Plasmid

   #228732

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D56R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

   Plasmid

   #228731

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CROPseq-Puro-2xMS2

   Plasmid

   #212627

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CROPseq-Puro-12xMS2

    Plasmid

    #212628

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CROPseq-Puro-4xMS2

    Plasmid

    #212626

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTBL3428 MTK234-LacO-BtoApegRNA

    Plasmid

    #226669

    Purpose

    MTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNA

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    hU6-LacOS-BtoApegRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-LacOS

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTBL3331 MTK234-TetO-AtoBpegRNA

    Plasmid

    #226668

    Purpose

    MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    hU6-TetO-AtoBpegRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-TetO

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTBL3241 PB-AtoBpegRNA-mCherry

    Plasmid

    #226666

    Purpose

    PiggyBac cargo vector encoding A to B pegRNA marked with mCherry

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    AtoB pegRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTBL3401 BtoApegRNA-sfGFP

    Plasmid

    #226667

    Purpose

    PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    BtoA pegRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-MCS-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225704

    Purpose

    Empty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1A

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-MCS-3AID-HA-2A-mCherryBSD

    Plasmid

    #225700

    Purpose

    Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1A

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-rTetR(SE)-VP64-2A-mCherryBSD

    Plasmid

    #225695

    Purpose

    Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    rTetR-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS3-tdc-1P::AI::acc-1::SL2::his-24::tagRFP::unc-54-3'UTR

    Plasmid

    #225926

    Purpose

    Expressing ACC-1 in RIM neuron

    Depositor

    Andrew Leifer

    Article

    Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

    Insert

    ACC-1 (acc-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    tdc-1

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR

    Plasmid

    #225925

    Purpose

    Expressing LGC-47 in RIM neuron

    Depositor

    Andrew Leifer

    Article

    Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

    Insert

    LGC-47 (lgc-47 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    tdc-1

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge