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Plasmid#120234PurposeCarries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing ermE*p promoter and ampicillin resistance gene
UseSynthetic BiologyTagsExpressionMutationbla (amp resistance gene)PromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK083 desmaMCS:minpromEGFP,exorh:mCherry
Plasmid#200016PurposeExpression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherryDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE
Plasmid#20944Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationH134RPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
p5E-pth2
Plasmid#191428PurposeTol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)DepositorInsertpth2 regulatory region (pth2 Zebrafish)
UseTol2 gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-KSENB-Nup133
Plasmid#87340PurposeTo express human Nup133 in mammalian cellsDepositorInsertNUP133 (NUP133 Human)
UseTagsEGFPExpressionMammalianMutationdeletion of Nup133 AA1-7PromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
opto-Tiam1
Plasmid#174508PurposeExpresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.DepositorInsertopto-Tiam1-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_Ub
Plasmid#172134PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_TOP-NLuc1.1_L5-L4
Plasmid#113867PurposeSingle pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.DepositorInsertsTOP (wnt dependent promoter)
NLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BZLF2
Plasmid#195784PurposeGateway Entry Clone of Epstein-Barr virus BZLF2DepositorInsertBZLF2 (BZLF2 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMito-MtrxRFP2
Plasmid#172324Purposefluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)DepositorInsertmitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
UseTagsHis-tag and Mitochondria localization sequenceExpressionMammalianMutationPromoterCMV, SP6Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-MXT GIRK2
Plasmid#20675DepositorInsertMus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
UseXenopus expressionTags3' UTR Xenopus B- globin and 5' UTR Xen…ExpressionMutationA369VPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA HisB TO rFez1
Plasmid#17385DepositorInsertFEZ1 (Fez1 Rat)
UseTagshis and mycExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
S1-Fc
Plasmid#164220PurposeExpresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporterDepositorInsertSpike S1 domain (S SARS-CoV)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BALF5
Plasmid#198577PurposeGateway Entry CloneDepositorInsertBALF5 (BALF5 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationNone (wildtype)PromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BALF2
Plasmid#198578PurposeGateway Entry CloneDepositorInsertBALF2 (BALF2 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationNone (wildtype)PromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSecTag-T210A-ATX
Plasmid#17840DepositorInsertT210A-ATX (ENPP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
791 p3E-2A-pCS2MCSpA v2
Plasmid#195973PurposeGateway p3E 2A fluorophoreDepositorInsert2A-multiple cloning site
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pRK5-HA-YFP-PRAS40
Plasmid#73389PurposeExpresses HA and YFP tagged PRAS40DepositorInsertAKT1 Substrate 1 (Proline-Rich) (AKT1S1 Human)
UseTagsHA and YFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only