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pCDB180

Plasmid

#80677

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

Depositor

David Baker

Publication

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Type

Empty backbone

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD-mhYFP

Plasmid

#186532

Purpose

Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-S147P/L195M/V206K

Promoter

araB

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-EPHB6

Plasmid

#23931

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHB6 (EPHB6 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX-ARF

Plasmid

#19929

Depositor

Yue Xiong

Publication

Zhang et al Cell. 1998 Mar 20. 92(6):725-34.

Insert

cyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)

Expression

Bacterial

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1

Plasmid

#10880

Depositor

Patrick Aebischer

Publication

Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

Insert

H1-shSOD1 (SOD1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
PB_SBE4:mScarlet-I-NLS

Plasmid

#154961

Purpose

encodes transcriptional reporter of SMAD2/3

Depositor

Ewa Bielczyk-Maczynska, Joshua Knowles

Publication

Taylor et al Sci Rep. 2020 Aug 21;10(1):14084. doi: 1

Insert

mScarlet

Use

Piggybac

Tags

NLS-PEST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-CALM1

Plasmid

#23768

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CALM1 (CALM1 Human)

Use

Gateway Cloning

Mutation

M74T V144E

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pEX-CMV-SP-STIM2(15-746)

Plasmid

#18868

Depositor

Tobias Meyer

Publication

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

STIM2 (STIM2 Human)

Expression

Mammalian

Mutation

Signal peptide(SP) 1-10 from STIM1 and residues 1…

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Publication

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Promoter

Cbh

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Publication

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mEos4a-C1

Plasmid

#54810

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Publication

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4a

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBCN22-R4R3

Plasmid

#26302

Depositor

Ben Lehner

Publication

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Type

Empty backbone

Expression

Worm

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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TU#710

Plasmid

#15278

Depositor

Martin Chalfie

Publication

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzgfp

Expression

Worm

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pMAX deltaN-ERG

Plasmid

#29447

Depositor

Valeri Vasioukhin

Publication

Klezovitch et al Proc Natl Acad Sci U S A. 2008 Feb 12. 1

Insert

ERG (ERG Human)

Expression

Mammalian

Mutation

Deletion of first 33 aa of ERG

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
APEX2-ChBP

Plasmid

#108878

Purpose

Expression of APEX2 fused to a nanobody against mCherry

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-ChBP

Expression

Mammalian

Available since

May 26, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - EGFP sgRNA 5

Plasmid

#51764

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Publication

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 5

Use

CRISPR and Lentiviral

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEXtev-HMG54kd

Plasmid

#26030

Depositor

Johann Deisenhofer

Publication

Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

Insert

3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)

Tags

GST

Expression

Bacterial

Mutation

Catalytic domain (amino acids 426-488) Methion…

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

rtTA

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR221-NR1H2

Plasmid

#79513

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR BETA (NR1H2 Human)

Use

Gateway Cloning

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (wt)

Plasmid

#48805

Purpose

expresses wt Akt1

Depositor

David Sabatini

Publication

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Tags

HA-GST-Presc

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article