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iRFP-N1
(Plasmid #54787)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 54787 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    iRFP-N1
  • Backbone size (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • iRFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • iRFP (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Excitation = 690; Emission = 713.

Codon usage for iRFP is different than the original Nature Biotechnology paper (PMID 21765402).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iRFP-N1 was a gift from Michael Davidson & Vladislav Verkhusha (Addgene plasmid # 54787 ; http://n2t.net/addgene:54787 ; RRID:Addgene_54787)
  • For your References section:

    Bright and stable near-infrared fluorescent protein for in vivo imaging. Filonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV. Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918. 10.1038/nbt.1918 PubMed 21765402