We narrowed to 14,886 results for: pET

1. pET28a-eGFP-Pep-H

   Plasmid

   #185788

   Purpose

   Encodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   eGFP-Pep4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET303-ColE1-TR-del1-40

   Plasmid

   #180236

   Purpose

   Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364)

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   Colicin E1
   

   Use

   Tags

   6x His-tag

   Expression

   Bacterial

   Mutation

   Truncation of ColE1 containing T and R domains (r…

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET303-ColE1-T-P110A

   Plasmid

   #180237

   Purpose

   Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   Colicin E1
   

   Use

   Tags

   6x His-tag

   Expression

   Bacterial

   Mutation

   Truncation of ColE1 containing T domain of ColE1 …

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b-MBP-bhaB5

   Plasmid

   #167812

   Purpose

   Expresses His6- and MBP-tagged BhaB5 in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

   Insert

   BhaB5
   

   Use

   Tags

   His6, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a(+)_co_L-HicDH

   Plasmid

   #185453

   Purpose

   CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   L-HicDH from Lactobacillus confusus DSM 20196
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30-T7-lacO-sfGFP

   Plasmid

   #180756

   Purpose

   pET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

   Depositor

   Michael Black

   Article

   Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

   Insert

   superfolder green fluorescent protein
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

   Plasmid

   #183387

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   redesigned Caspase-4 (AA94-377) (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   ANR261ENP, D315H, M318K, T352K

   Promoter

   T7

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-11-LECT2-V40

   Plasmid

   #183031

   Purpose

   Expression of LECT2 V40 variant protein from T7 promoter

   Depositor

   Gareth Morgan

   Article

   Belonogov et al bioRxiv 2022.03.01.482540

   Insert

   LECT2 (LECT2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V40 variant

   Promoter

   T7

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

   Plasmid

   #169664

   Purpose

   Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / RPBSA

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid

    #169657

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid

    #169659

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-SU(Z)12(624-900)-H6

    Plasmid

    #183772

    Purpose

    Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

    Insert

    SU(Z)12 residues 624-900 (Su(z)12 Fly)
    

    Use

    Tags

    His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM-60-NusA-Dmp68_B

    Plasmid

    #145966

    Purpose

    Bacterial Expression of Dmp68

    Depositor

    Elisa Izaurralde

    Insert

    Dmp68 (Rm62 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a Cdk2ap1ΔN (MT2B2)

    Plasmid

    #178034

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    First N-Terminal 27aa are deleted

    Promoter

    T7LacI

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6xHis-CPL2/pET28a

    Plasmid

    #172095

    Purpose

    cDNA of CER11/CPL2 cloned into pET28a for protein expression

    Depositor

    George Haughn

    Article

    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

    Insert

    CPL2 (CPL2 Mustard Weed)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET28_6xHis-PARP1delZn1-L713F

    Plasmid

    #173937

    Purpose

    Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn1-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 1-96; L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_6xHis-PARP1delZn3-L713F

    Plasmid

    #173934

    Purpose

    Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn3-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 214-373; L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28_6xHis-PARP1-G871L

    Plasmid

    #174798

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G871L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G871L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28_6xHis-PARP1-A870L

    Plasmid

    #174797

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A870L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A870L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_6xHis-PARP1-K893I

    Plasmid

    #174799

    Purpose

    Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-K893I (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K893I, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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