We narrowed to 9,801 results for: pET

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pET28a(+)/FbGH30

Plasmid

#86463

Purpose

Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FbGH30

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS

Plasmid

#85734

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

Depositor

Gregory Poon

Article

Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

Insert

ETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)

Tags

6xHis following thrombin cleavage site LVPR|G

Expression

Bacterial

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN-01*

Plasmid

#80423

Purpose

For expression of EPN-01* in E. coli

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Tags

Myc

Expression

Bacterial

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only
pETM6-H9-mCherry

Plasmid

#66532

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H9

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H9

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET3a RegIIIg

Plasmid

#64936

Purpose

bacterial expression of processed mouse RegIIIgamma

Depositor

Lora Hooper

Article

Mukherjee et al J Biol Chem. 2009 Feb 20;284(8):4881-8.

Insert

RegIIIg (Reg3g Mouse)

Expression

Bacterial

Mutation

processed form

Promoter

T7

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
YPet-Lysosomes-20

Plasmid

#56636

Purpose

Localization: Lysosome Membrane, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

LAMP1

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
YPet-LaminB1-10

Plasmid

#56635

Purpose

Localization: Nuclear Envelope, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

LaminB1 (LMNB1 Human)

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
YPet-Mito-7

Plasmid

#56637

Purpose

Localization: Mitochondria, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET29b-ClbP

Plasmid

#48244

Purpose

Untagged, full-length protein for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Tags

None

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Article

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Tags

His and thrombin cleavage

Expression

Bacterial

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pET3a-AqAdk_Y52C

Plasmid

#18090

Depositor

Dorothee Kern

Article

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad A. aeolicus)

Expression

Bacterial

Mutation

Tyr 52 changed to Cys

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
peT3a-AqAdk_MVGDH

Plasmid

#18092

Depositor

Dorothee Kern

Article

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad A. aeolicus)

Tags

6xHis

Expression

Bacterial

Mutation

Tyr 52 changed to Cys, Val 145 changed to Cys, …

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
PET11a-Af2sir2

Plasmid

#8383

Depositor

Cynthia Wolberger

Article

Avalos et al Mol Cell 2002 Sep;10(3):523-35.

Insert

Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)

Expression

Bacterial

Mutation

PET11a-Af2Sir2D103N

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pET32b-MED19

Plasmid

#15438

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

His and Trx

Expression

Bacterial

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pETMutS-E41A

Plasmid

#13048

Depositor

Peggy Hsieh

Article

Schofield et al J Biol Chem. 2001 Dec 7. 276(49):45505-8

Insert

MutS

Expression

Bacterial

Mutation

mutation Glu41 to Ala full-length

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pET28b-ULP1-405-621

Plasmid

#229224

Purpose

For expression of ULP1 protein

Depositor

Andy LiWang

Insert

ULP1 (ULP1 Budding Yeast)

Expression

Bacterial

Mutation

405-621 (truncated)

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Mucin MUC1-5TR-pET15b

Plasmid

#174694

Purpose

Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

Depositor

Jean Gariepy

Article

Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

Insert

MUC1-TR

Tags

hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET32a-Trx-His6-TEV_SacB

Plasmid

#164588

Purpose

Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.

Depositor

Ernst Schonbrunn

Insert

TEV-SacB

Tags

Trx-His6 followed by TEV site

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB3-mCherry-6xHis

Plasmid

#130985

Purpose

Bacterial expression of mouse EB3-mCherry-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB3-mCherry-6xHis (Mapre3 Mouse)

Tags

mCherry-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB3-eGFP-6xHis

Plasmid

#130983

Purpose

Bacterial expression of mouse EB3-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB3-GFP-6xHis (Mapre3 Mouse)

Tags

eGFP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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