pET14b_SG
(Plasmid #59737)

• Purpose: Expresses SH3-GUK domains of human ZO-1
• Depositing Lab: Alan Fanning
• Publication: Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-17. doi: 10.1074/jbc.M109.093674. Epub 2010 Mar 3.

Backbone

• Vector backbone: pET14b
• Backbone manufacturer: EMD Millipore
• Backbone size w/o insert (bp): 4671
• Total vector size (bp): 5553
• Modifications to backbone: Thrombin cleavage site of pET14b replaced by TEV protease cleavage site.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: zonula occludens-1
• Alt name: TJP-1
• Species: H. sapiens (human)
• Insert Size (bp): 882
• Entrez Gene: TJP1 (a.k.a. ZO-1)
• Tags / Fusion Proteins:
  • HIS tag (N terminal on backbone)
  • thrombin clvg (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde I (not destroyed)
• 3′ cloning site: Bam HI (not destroyed)
• 5′ sequencing primer: T7 fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry