pTRE ZO1myc
(Plasmid
#30318)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTRE
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3100
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Vector typeMammalian Expression
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Selectable markersNONE
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZonula Occludens-1
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Alt nameTJP1
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SpeciesH. sapiens (human)
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Insert Size (bp)5376
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Entrez GeneTJP1 (a.k.a. ZO-1)
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eco RI (not destroyed)
- 3′ cloning site Eco RI (not destroyed)
- 5′ sequencing primer GACCTCCATAGAAGACACC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRE ZO1myc was a gift from Alan Fanning (Addgene plasmid # 30318 ; http://n2t.net/addgene:30318 ; RRID:Addgene_30318) -
For your References section:
The unique-5 and -6 motifs of ZO-1 regulate tight junction strand localization and scaffolding properties. Fanning AS, Little BP, Rahner C, Utepbergenov D, Walther Z, Anderson JM. Mol Biol Cell. 2007 Mar . 18(3):721-31. 10.1091/mbc.e06-08-0764 PubMed 17182847