We narrowed to 7,293 results for: 11

1. pEasyG1_nat

   Plasmid

   #184911

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3538 pMIG Bad

   Plasmid

   #8787

   Depositor

   Stanley Korsmeyer

   Article

   Cheng et al Mol Cell 2001 Sep;8(3):705-11.

   Insert

   Bad (Bad Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pCFJ1208

   Plasmid

   #44490

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
   

   Use

   Gateway expression clone

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJH2217

   Plasmid

   #179251

   Purpose

   Psra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

   Depositor

   Mei Zhen

   Article

   Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

   Insert

   TWK-18 (twk-18 Nematode)
   

   Tags

   wCherry

   Expression

   Worm

   Mutation

   M280I

   Promoter

   Psra-11

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-BBS4

   Plasmid

   #218713

   Purpose

   Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

   Insert

   BBS4 (BBS4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   I354T (t1110c)

   Promoter

   CMV

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGSTag Flag Jnk1a1

   Plasmid

   #15743

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk1a1 (MAPK8 Human)
   

   Tags

   Flag

   Expression

   Bacterial

   Available Since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

7. p1977 (pUC19-PTDH3-PTEF1)

   Plasmid

   #78230

   Purpose

   Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   Bidirectional promoters pTDH3-pTEF1
   

   Expression

   Bacterial

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-C1-hEFA6A

   Plasmid

   #206412

   Purpose

   Mammalian expression of hEFA6A

   Depositor

   Kazuhisa Nakayama, Hye-Won Shin

   Article

   Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

   Insert

   EFA6A (PSD Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 Flag Jnk1b2

   Plasmid

   #13752

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk1b2 (MAPK8 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pEG BacMam C term 3C eGFP myc

    Plasmid

    #160685

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    His8 eGFP 3C

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-NES-ZapCmR2

    Plasmid

    #59015

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pGSTag Flag Jnk3a2

    Plasmid

    #15748

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk3a2 (MAPK10 Human)
    

    Expression

    Bacterial

    Available Since

    March 6, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pGL3-NRASlong

    Plasmid

    #13797

    Depositor

    Frank Slack

    Article

    Johnson et al Cell. 2005 Mar 11. 120(5):635-47.

    Insert

    NRAS (NRAS Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

14. FL-fibulin-3 pcDNA4

    Plasmid

    #29700

    Depositor

    Mariano Viapiano

    Article

    Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

    Insert

    EFEMP1 (EFEMP1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172504

    Purpose

    Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 Flag Jnk2b2

    Plasmid

    #13757

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2b2 (MAPK9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pET22b-T5-FB-E25TAG-F6C

    Plasmid

    #176546

    Purpose

    Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

    Depositor

    Han Xiao

    Article

    Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

    Insert

    IgG affinity protein
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    change phenylalanine 6 to cysteine; change glutam…

    Promoter

    T5

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEBG-Pak1 83-149 L107F

    Plasmid

    #12215

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Autoinhibitory domain (aa 83-149) with L107F muta…

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pOET3-N-TEV-Twin-Strep

    Plasmid

    #203323

    Purpose

    Generate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Tags

    TEV cleavable site (ENLYFQG) and Twin-strep-tag

    Expression

    Insect

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pconst #4-RFP

    Plasmid

    #81078

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Promoter

    Constitutive Promoter - BBa_J23107

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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