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We narrowed to 4,689 results for: crispr c plasmids

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Showing: 4381 - 4410 of 4689 results

  8. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid
    #181743
    Purpose
    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181743-plasmid-map-sequence-id-354675

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  9. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Mutation
    The second base of the first intron of B2M (From …
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215546-plasmid-map-sequence-id-425336

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  10. B2M-SDMutation-EPG

    Plasmid
    #215545
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    Use
    CRISPR
    Mutation
    The second base of the first intron of B2M (From …
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215545-plasmid-map-sequence-id-425338

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  11. pYPQ233-STU

    Plasmid
    #138111
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    dLbCas12a-SRDX
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138111-plasmid-map-sequence-id-272043

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  13. pBFC0996

    Plasmid
    #186695
    Purpose
    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186695-plasmid-map-sequence-id-371382

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  14. pYPQ284-RVRR

    Plasmid
    #160327
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V, N573R and K625R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Mb2Cas12a RVRR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N563R, K569V, N573R and K625R
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    160327-plasmid-map-sequence-id-317695

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  15. AAV-KrasWT/sgKras/Cre

    Plasmid
    #99848
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99848-plasmid-map-sequence-id-193786

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  17. pYPQ284-v2

    Plasmid
    #138119
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Mb2Cas12a v2
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    D172R, N563R and K569R
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138119-plasmid-map-sequence-id-272076

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  18. pYPQ284-RVR

    Plasmid
    #138117
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Mb2Cas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N563R, K569V and N573R
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138117-plasmid-map-sequence-id-272074

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  19. pYPQ289-RVR

    Plasmid
    #138127
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    ErCas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    D513R, K519V and N523R
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138127-plasmid-map-sequence-id-272088

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  20. pYPQ284-v1

    Plasmid
    #138118
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R mutation, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Mb2Cas12a v1
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    D172R
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138118-plasmid-map-sequence-id-272075

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  22. pYPQ223-STU

    Plasmid
    #138109
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminator
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    dAsCas12a-SRDX
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138109-plasmid-map-sequence-id-272024

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  28. pYPQ287-RVR

    Plasmid
    #138124
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    BsCas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N512R, K518V and N522R
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138124-plasmid-map-sequence-id-272082

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  29. pYPQ285-RVR

    Plasmid
    #138121
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a with N512R, K518V and N522R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Lb5Cas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N512R, K518V and N522R
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138121-plasmid-map-sequence-id-272079

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Showing: 4381 - 4410 of 4689 results