We narrowed to 4,183 results for: biorxiv

1. CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

   Plasmid

   #200793

   Purpose

   Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   CLIPf and SNAPf, HisTag, FLAG

   Expression

   Mammalian

   Mutation

   1-914; Δ505-610

   Promoter

   CMV & T7

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TMEM16A-I547K-peGFP-N1

   Plasmid

   #228583

   Purpose

   Expresses mouse TMEM16A-I547K-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I547K

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TMEM16A-E551K-peGFP-N1

   Plasmid

   #228584

   Purpose

   Expresses mouse TMEM16A-E551K-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E551K

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E-CAGGS (JDW 912)

   Plasmid

   #224472

   Purpose

   Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   CAGGS Promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCKC014

   Plasmid

   #226623

   Purpose

   pLenti wt hTdT template for library 3

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Insert

   Terminal deoxynucleotidyl transferase (DNTT Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5E_hs_Glast (JDW 1182)

   Plasmid

   #224483

   Purpose

   Gateway compatible 5' entry clone containing the Human GLAST promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   GLAST promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-EF1a (JDW 1164)

   Plasmid

   #224473

   Purpose

   Gateway compatible 5' entry clone containing the human EF1a promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   EF1a promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME AmCyan (JDW 1151)

   Plasmid

   #224485

   Purpose

   Gateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   AmCyan
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-LexA-mODC (no LexOp) (JDW 937)

   Plasmid

   #224504

   Purpose

   Gateway compatible middle entry clone containing a destabilized LexA transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   LexA-mODC
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-myc-BirA (JDW 1210)

    Plasmid

    #224509

    Purpose

    Gateway compatible middle entry clone containing a Myc tagged BirA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Myc-BirA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-V5-mTagBFP2 (JDW 871)

    Plasmid

    #224531

    Purpose

    A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    V5-mTagBFP2
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

    Plasmid

    #194217

    Purpose

    Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    UAS-ybTATA-tagBFP; PGK-mCitrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    GAL4 UAS-ybTATA; PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LCB1_v1.3 (MTK 3a) (pZYW044)

    Plasmid

    #194227

    Purpose

    Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    LCB1_v1.3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LCB3_v1.2 (MTK 3a) (pZYW045)

    Plasmid

    #194228

    Purpose

    Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    LCB3_v1.2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. WPRE (MTK 4b) (pAN2410)

    Plasmid

    #194235

    Purpose

    Mammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbones

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    WPRE terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

    Plasmid

    #223163

    Purpose

    2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG

    Plasmid

    #217717

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR1 (CELSR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2453

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. FKBP-LBR(1-245)-GFP

    Plasmid

    #222425

    Purpose

    To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al bioRxiv 2024.05.31.596797

    Insert

    LBR(1-245aa) (LBR Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Truncation (Includes aa 1-245)

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-EF1A-Puro-mCherry-RAB7-S72A

    Plasmid

    #221556

    Purpose

    Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Change serine 72 to alanine

    Promoter

    EF1a

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

    Plasmid

    #221552

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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